Journal of Lipid Research, Vol 26, 1444-1454, Copyright © 1985 by Lipid Research, Inc.
Dietary and species influence on potential of plasma to stimulate differentiation and lipid accumulation in cultured adipocyte precursors
P Bjorntorp, IM Faust, WH Miller Jr, M Karlsson, G Sypniewska and K Dahlgren
Sera and plasma from different species and from rats of various dietary
statuses were compared with regard to effects on proliferation,
glycerophosphate dehydrogenase (GPDH) activity, and lipid-filing of rat
adipocyte precursors converting to adipocytes in primary cell culture. All
of the tested sera and plasma samples were comparably supportive of cell
multiplication, but their effects on elevation of GPDH activity (a key
event in adipocyte differentiation) and lipid-filling varied greatly.
Plasma supported a much greater increase in GPDH activity than serum, while
serum from cats supported a much lower increase than serum from humans,
calves, goats, or rats. Dietary status of rats did not affect the potential
of plasma to support GPDH activity, but did affect plasma support of
lipid-filing. A higher than normal degree of lipid- filling was promoted by
plasma from rats fed a high-fat, high-sugar diet, while a lower than normal
degree was promoted by plasma from fasted rats. Lipid-filling was also
found to vary in response to changes in content of very low density
lipoprotein (VLDL) in human plasma. This suggests that the influence of
diet on the potential of plasma to promote adipocyte lipid-filling may be
mediated by the effect of diet on plasma VLDL. The absence of a
diet-dependent effect of plasma either on multiplication of adipocyte
precursors or on degree of elevation of GPDH activity leaves unresolved the
mechanism by which diet affects adipocyte production in animals.
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