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Journal of Lipid Research, Vol 26, 267-269, Copyright © 1985 by Lipid Research, Inc.

ARTICLES

Latex immunoassay of human serum Lp(a+) lipoprotein

N Vu-Dac, A Chekkor, H Parra, P Duthilleul and JC Fruchart

A sensitive latex immunoassay for human serum lipoprotein Lp(a+) is based on direct agglutination by Lp(a+) of latex particles coated with specific antibody. The agglutination is quantified by turbidimetry using a photometer at 360 nm. The stabilization of antibody-coated latex particles by bovine serum albumin occurs under well-defined conditions (pH, concentration of bovine serum albumin, and antibody loading of latex particles). The standard curve of serum lipoprotein Lp(a+) ranges from 0.05 to 1.15 mg/l. Inter- and intra-assay coefficients of variation were less than 8% and 3%, respectively. Results were well correlated with those obtained by electroimmunodiffusion (r = 0.98, n = 108).
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