Journal of Lipid Research, Vol 26, 351-359, Copyright © 1985 by Lipid Research, Inc.
Plasma lipoprotein changes attending the intravenous administration of Triton WR-1339 in normolipidemic dogs: preferential effect on high density lipoproteins
C Edelstein, RE Byrne, K Yamamoto, C Zarins and AM Scanu
The nonionic detergent Triton WR-1339 was injected intravenously into
normolipidemic dogs in a single dose of 150 mg/kg body weight followed by
three other injections (75 mg/kg) on days 2, 6, and 12. The Triton produced
a significant elevation of the plasma cholesterol of these animals, but not
of their triglyceride levels, and profound changes of their plasma
lipoproteins, particularly of the high density lipoprotein class. These
changes were dependent on the concentration of Triton attained in plasma;
when the levels were above 1.5 mg/ml, density gradient ultracentrifugation,
electrophoretic, and chemical analyses indicated that an interaction
between Triton and HDL had occurred. This interaction was attended by a
gradual loss of the surface components of HDL, namely apoA-I,
phospholipids, and unesterified cholesterol, and by the appearance of two
cholesteryl ester-rich lipoproteins of d 1.019- 1.024 g/ml and d
1.038-1.058 g/ml containing apoA-I and proteins with electrophoretic
mobilities of apoB, apoE, and apoA-IV. At the time that these changes had
occurred, the activities of the enzymes lecithin: cholesterol
acyltransferase and post-heparin lipase were unaffected. When 125I-labeled
apoA-I was injected intravenously into animals receiving Triton, the
residence time of the radiolabeled protein in plasma increased from a
control value of 3.1 days to 7.2 days. However, the apparent half-times of
the radiolabeled apoA-I varied among the lipoprotein fractions it was
associated with: d 1.119-1.159 g/ml, 5.28 days; d 1.019-1.024 g/ml, 7.55
days, and d 1.038-1.058 g/ml, 5.39 days.(ABSTRACT TRUNCATED AT 250 WORDS)
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