HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Christie, W. W. Search for Related Content PubMed PubMed Citation Articles by Christie, W. W. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 26, 507-512, Copyright © 1985 by Lipid Research, Inc.

ARTICLES

Rapid separation and quantification of lipid classes by high performance liquid chromatography and mass (light-scattering) detection

WW Christie

The major lipid classes in animal tissues, varying in polarity from cholesteryl esters to lysophosphatidylcholine, can be separated and accurately quantified by high performance liquid chromatography on a short 3-mu silica column and using a mass (light-scattering) detector. Sample sizes of 0.2 to 0.4 mg are optimum and the analysis is completed in only 20 min. The column is reactivated and ready for the next analysis after a further 10 min. After acid treatment, the plasmalogen forms of phospholipids can be determined. Applications of the procedure to the analysis of rat liver, heart, erythrocytes, and plasma lipids are described.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Koutsari, D. A. Dumesic, B. W. Patterson, S. B. Votruba, and M. D. Jensen Plasma Free Fatty Acid Storage in Subcutaneous and Visceral Adipose Tissue in Postabsorptive Women Diabetes, May 1, 2008; 57(5): 1186 - 1194. [Abstract] [Full Text] [PDF]

				S. Shadid, C. Koutsari, and M. D. Jensen Direct Free Fatty Acid Uptake Into Human Adipocytes In Vivo: Relation to Body Fat Distribution Diabetes, May 1, 2007; 56(5): 1369 - 1375. [Abstract] [Full Text] [PDF]

				E. Persson, L. Lofgren, G. Hansson, B. Abrahamsson, H. Lennernas, and R. Nilsson Simultaneous assessment of lipid classes and bile acids in human intestinal fluid by solid-phase extraction and HPLC methods J. Lipid Res., January 1, 2007; 48(1): 242 - 251. [Abstract] [Full Text] [PDF]

				M. D. Jensen, M. G. Sarr, D. A. Dumesic, P. A. Southorn, and J. A. Levine Regional uptake of meal fatty acids in humans Am J Physiol Endocrinol Metab, December 1, 2003; 285(6): E1282 - E1288. [Abstract] [Full Text] [PDF]

				L. L. W. Cooling, D. S. Zhang, S. J. Naides, and T. A. W. Koerner Glycosphingolipid expression in acute nonlymphocytic leukemia: common expression of shiga toxin and parvovirus B19 receptors on early myeloblasts Blood, January 15, 2003; 101(2): 711 - 721. [Abstract] [Full Text] [PDF]

				Z. Guo, P. Mishra, and S. Macura Sampling the intramyocellular triglycerides from skeletal muscle J. Lipid Res., July 1, 2001; 42(7): 1041 - 1048. [Abstract] [Full Text] [PDF]

				K. L. Krenzer, M. Reza Dana, M. D. Ullman, J. M. Cermak, D. B. Tolls, J. E. Evans, and D. A. Sullivan Effect of Androgen Deficiency on the Human Meibomian Gland and Ocular Surface J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4874 - 4882. [Abstract] [Full Text]

				Z. Guo, B. Burguera, and M. D. Jensen Kinetics of intramuscular triglyceride fatty acids in exercising humans J Appl Physiol, November 1, 2000; 89(5): 2057 - 2064. [Abstract] [Full Text] [PDF]

				D. A. Sullivan, B. D. Sullivan, M. D. Ullman, E. M. Rocha, K. L. Krenzer, J. M. Cermak, I. Toda, M. G. Doane, J. E. Evans, and L. A. Wickham Androgen Influence on the Meibomian Gland Invest. Ophthalmol. Vis. Sci., November 1, 2000; 41(12): 3732 - 3742. [Abstract] [Full Text]

				S. A. Romanski, R. M. Nelson, and M. D. Jensen Meal fatty acid uptake in adipose tissue: gender effects in nonobese humans Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E455 - E462. [Abstract] [Full Text] [PDF]

				Z. Guo and M. D. Jensen Blood Glycerol Is an Important Precursor for Intramuscular Triacylglycerol Synthesis J. Biol. Chem., August 20, 1999; 274(34): 23702 - 23706. [Abstract] [Full Text] [PDF]

				J. F. H. M. Brouwers, E. A. A. M. Vernooij, A. G. M. Tielens, and L. M. G. van Golde Rapid separation and identification of phosphatidylethanolamine molecular species J. Lipid Res., January 1, 1999; 40(1): 164 - 169. [Abstract] [Full Text]

				D. L. Lankester, A. M. Brown, and V. A. Zammit Use of cytosolic triacylglycerol hydrolysis products and of exogenous fatty acid for the synthesis of triacylglycerol secreted by cultured rat hepatocytes J. Lipid Res., September 1, 1998; 39(9): 1889 - 1895. [Abstract] [Full Text]

				R. Homan and K. L. Hamelehle Phospholipase A2 relieves phosphatidylcholine inhibition of micellar cholesterol absorption and transport by human intestinal cell line Caco-21 J. Lipid Res., June 1, 1998; 39(6): 1197 - 1209. [Abstract] [Full Text]

				Z. Guo and M. D. Jensen Intramuscular fatty acid metabolism evaluated with stable isotopic tracers J Appl Physiol, May 1, 1998; 84(5): 1674 - 1679. [Abstract] [Full Text] [PDF]