Journal of Lipid Research, Vol 26, 513-514, Copyright © 1985 by Lipid Research, Inc.
Alkaline phosphatase activity can interfere with the enzymatic determination of triglycerides
GJ Dombrowski Jr, KR Swiatek and KL Chao
Unlike human plasma, rat plasma was found to contain substantial quantities
of alkaline phosphatase. The large amount of phosphatase activity in rat
plasma interferes with assay methods that employ orthophosphoric acid
esters as substrates or reaction intermediates. Although these methods are
effective when applied to human plasma samples, they cannot be used with
rodent plasma.