HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dory, L. Articles by Roheim, P. S. Search for Related Content PubMed PubMed Citation Articles by Dory, L. Articles by Roheim, P. S. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 26, 519-527, Copyright © 1985 by Lipid Research, Inc.

ARTICLES

Heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoproteins: implications for a role in reverse cholesterol transport

L Dory, LM Boquet, RL Hamilton, CH Sloop and PS Roheim

The heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoprotein (HDL) was investigated and compared to plasma HDL. Interstitial fluid and plasma HDL of normal and cholesterol-fed dogs was subfractionated by ultracentrifugation and affinity and molecular weight sieving chromatography. Both plasma (P) and interstitial fluid (L) HDL can be subfractionated into a larger fraction (P-I and L-I) and a smaller one (P-II and L-II). Cholesterol feeding induces a large increase in the P-I and L-I component of HDL, but the increase in L-I is far greater in proportion than that of P-I. Furthermore, L-I of cholesterol-fed dogs appears to be almost exclusively discoid in shape, while only approximately 15% of particles in P-I are discoidal. The discoid HDL of L-I is reflected in its chemical composition: 28% unesterified cholesterol, 6% cholesteryl ester, 45% phospholipid, and 21% protein. It contains large amounts of apoE in addition to apoA-I and apoA-IV. We found that the association of apoE with discoid particles is frequent, but not necessary. Calculations based on known protein mass and quantitation of discoid particles on electron micrographs suggest that the concentration of discoid particles in the peripheral lymph of cholesterol-fed dogs is about fourfold that of the plasma of the same animal. These findings provide strong circumstantial evidence for the peripheral formation of discoid HDL, perhaps as an early event in reverse cholesterol transport.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M.-D. Wang, R. S. Kiss, V. Franklin, H. M. McBride, S. C. Whitman, and Y. L. Marcel Different cellular traffic of LDL-cholesterol and acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways J. Lipid Res., March 1, 2007; 48(3): 633 - 645. [Abstract] [Full Text] [PDF]

				T. Kujiraoka, M. N. Nanjee, T. Oka, M. Ito, M. Nagano, C. J. Cooke, S. Takahashi, W. L. Olszewski, J. S. Wong, I. P. Stepanova, et al. Effects of Intravenous Apolipoprotein A-I/Phosphatidylcholine Discs on LCAT, PLTP, and CETP in Plasma and Peripheral Lymph in Humans Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1653 - 1659. [Abstract] [Full Text] [PDF]

				M. N. Nanjee, C. J. Cooke, J. S. Wong, R. L. Hamilton, W. L. Olszewski, and N. E. Miller Composition and ultrastructure of size subclasses of normal human peripheral lymph lipoproteins: quantification of cholesterol uptake by HDL in tissue fluids J. Lipid Res., April 1, 2001; 42(4): 639 - 648. [Abstract] [Full Text]

				M. N. Nanjee, C. J. Cooke, W. L. Olszewski, and N. E. Miller Concentrations of Electrophoretic and Size Subclasses of Apolipoprotein A-I-Containing Particles in Human Peripheral Lymph Arterioscler. Thromb. Vasc. Biol., September 1, 2000; 20(9): 2148 - 2155. [Abstract] [Full Text] [PDF]

				Y. Stein, O. Stein, N. Duverger, G. Halperin, Y. Dabach, G. Hollander, and M. Ben-Naim Clearance of Cationized LDL Cholesterol From a Muscle Depot Is Not Enhanced in Human Apolipoprotein A-IV Transgenic Mice Arterioscler. Thromb. Vasc. Biol., January 1, 2000; 20(1): 179 - 184. [Abstract] [Full Text] [PDF]

				S. Braschi, T. A-M. Neville, M.-C. Vohl, and D. L. Sparks Apolipoprotein A-I charge and conformation regulate the clearance of reconstituted high density lipoprotein in vivo J. Lipid Res., March 1, 1999; 40(3): 522 - 532. [Abstract] [Full Text]

				R. J. McCombs, D. E. Marcadis, J. Ellis, and R. B. Weinberg Attenuated Hypercholesterolemic Response to a High-Cholesterol Diet in Subjects Heterozygous for the Apolipoprotein A-IV-2 Allele N. Engl. J. Med., September 15, 1994; 331(11): 706 - 710. [Abstract] [Full Text]