HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Duvic, C. R. Articles by Thompson, J. J. Search for Related Content PubMed PubMed Citation Articles by Duvic, C. R. Articles by Thompson, J. J. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 26, 540-548, Copyright © 1985 by Lipid Research, Inc.

ARTICLES

Identification of a mouse monoclonal antibody, LHLP-1, specific for human Lp(a)

CR Duvic, G Smith, WE Sledge, LT Lee, MD Murray, PS Roheim, WR Gallaher and JJ Thompson

Heretofore, immunologic reagents used to define and quantify human Lp(a) have been polyclonal in origin and therefore heterogeneous in antigenic specificity. We report here the isolation of a mouse monoclonal antibody, LHLP-1, monospecific for Lp(a). The antigen reactive with LHLP-1 was expressed in both lipoprotein Lp(a) as well as apolipoprotein Lp(a) delipidated by SDS treatment; however, disulfide reduction of apolipoprotein Lp(a) inhibited LHLP-1 reactivity. The antigen reactive with LHLP-1 on Lp(a), therefore, appears not to require lipid for expression of its conformationally dependent (disulfide-inhibitable) epitope. Antigen reactivity was virtually absent in the apoB and other proteins contained in very low density, low density, and high density lipoprotein particles. Immunologic quantification of Lp(a) in individual serum samples with a rabbit reference antiserum or LHLP-1 showed good correlation. We conclude that the monoclonal antibody LHLP-1 identifies an antigen unique to Lp(a) and that this antibody may therefore be useful in the further characterization and measurement of human Lp(a).
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. L. Rainwater, C. A. McMahan, G. T. Malcom, W. D. Scheer, P. S. Roheim, H. C. McGill Jr, and J. P. Strong Lipid and Apolipoprotein Predictors of Atherosclerosis in Youth : Apolipoprotein Concentrations Do Not Materially Improve Prediction of Arterial Lesions in PDAY Subjects Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 753 - 761. [Abstract] [Full Text] [PDF]