J. Lipid Res.
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Journal of Lipid Research, Vol 26, 549-555, Copyright © 1985 by Lipid Research, Inc.


ARTICLES

Apolipoprotein A-I-containing lipoproteins with pre-beta electrophoretic mobility

ST Kunitake, KJ La Sala and JP Kane

Among the apoA-I-containing lipoproteins isolated by selected-affinity immunosorption from human serum and plasma, we have identified a subpopulation which, unlike the bulk of high density lipoproteins, has pre-beta electrophoretic mobility. This pre-beta subpopulation can be observed directly in fresh plasma by immunoelectrophoresis. It contains phospholipid and free and esterified cholesterol, but protein constitutes 90% of its mass. Apolipoprotein A-I is the predominant apolipoprotein in this subpopulation; apolipoprotein A-II and the B lipoproteins are not detected. The protein moiety of this subpopulation exhibits markedly lower helicity than that of high density lipoproteins isolated by ultracentrifugation.
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