Journal of Lipid Research, Vol 26, 726-734, Copyright © 1985 by Lipid Research, Inc.
Alterations in the metabolism of lipids in ischemia of the liver and kidney
SD Finkelstein, D Gilfor and JL Farber
The metabolism of lipids in the ischemic liver has been examined in the
attempt to define the cause of the previously described loss of
phospholipid and to determine whether additional alterations occur that may
be related to the disturbances in membrane function. With 3 hr of ischemia,
30% of the cellular phospholipid was lost when measured either as phosphate
in a lipid extract of the whole liver or as fatty acyl esters after
separation by thin-layer chromatography of the major lipid classes in the
same extracts. All phospholipid species were equally affected, and there
was no accumulation of lysophospholipids. The loss of phospholipid acyl
chains was not accompanied by an increased number of acyl esters as mono-,
di-, or triglycerides. There was no increase in the size of the free fatty
acid pool, and the content of long chain acyl CoA esters decreased by 50%.
The acyl chain composition of the free fatty acid and neutral lipid pools
changed, however, to resemble more closely that of the phospholipids. There
was no change in the fatty acid composition of the phospholipids. The
incorporation of intraportally injected [3H]arachidonic acid into total
phospholipids was decreased upon reperfusion of liver that had been
ischemic for only 20 min. These data are consistent with a loss of fatty
acyl chains from the phospholipids into the free fatty acid pool. A few of
these chains are incorporated into neutral lipids, but most are lost from
the liver.(ABSTRACT TRUNCATED AT 250 WORDS)
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