Journal of Lipid Research, Vol 26, 860-866, Copyright © 1985 by Lipid Research, Inc.
Characterization of neutral sphingolipids from chicken erythrocytes
T Shiraishi and Y Uda
The neutral sphingolipids from chicken erythrocytes were characterized. The
total concentration of neutral sphingolipids was found to be 480 nmol/g of
dry stroma. They were isolated and purified by droplet counter-current
chromatography, Iatrobeads column chromatography, and preparative
thin-layer chromatography. The major neutral sphingolipids were free
ceramide, ceramide monohexoside, ceramide dihexoside, and ceramide
pentahexoside, which represented 43%, 23.5%, 10.0%, and 3.6% of the long
chain bases, respectively. Thus, free ceramide was the most abundant
neutral sphingolipid in chicken erythrocytes. Ceramide monohexoside was
composed of more galactosylceramide than glucosylceramide.
Galabiosylceramide was found in the ceramide dihexoside fraction together
with lactosylceramide. Ceramide pentahexoside was a Forssman glycolipid.
There were two groups of neutral sphingolipids; one had mainly C16 fatty
acid and the other had C22 and C24 fatty acids. In both groups sphingosine
(d18:1) was predominant as a long chain base. 2-Hydroxy-C16 fatty acid was
a major component of one of the ceramide monohexosides.
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