HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Jackson, E. M. Articles by Mott, G. E. Search for Related Content PubMed PubMed Citation Articles by Jackson, E. M. Articles by Mott, G. E. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 26, 893-897, Copyright © 1985 by Lipid Research, Inc.

ARTICLES

Fractionation of fecal neutral steroids by high performance liquid chromatography

EM Jackson, CA Kloss, ST Weintraub and GE Mott

Fecal neutral steroids were fractionated by high performance liquid chromatography (HPLC) into three major fractions: 5 beta-H, 3-keto steroids; 5 beta-H, 3 beta-hydroxy steroids; and 5 alpha-H and delta 5- 3 beta-hydroxy steroids. This separation was achieved in about 10 minutes, with greater than 97% recovery of standards in each fraction. Gas-liquid chromatographic quantitation of fecal steroids fractionated by either HPLC or thin-layer chromatography gave nearly identical results. A method using both C18 reverse phase and silica HPLC to purify radiolabeled sterols is also described.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?