Journal of Lipid Research, Vol 26, 1166-1169, Copyright © 1985 by Lipid Research, Inc.
A sensitive method for the quantitation of lysophosphatidylcholine in canine heart
M Wientzek, G Arthur, RY Man and PC Choy
We have developed a procedure for the determination of small amounts of
lysophosphatidylcholine in cardiac tissue. Lysophosphatidylcholine from
canine heart was separated from the major phospholipids by column
chromatography, and then acetylated with labeled acetic anhydride. The
acetylated lysophosphatidylcholine was isolated by thin-layer
chromatography and the lysophosphatidylcholine content was calculated from
the radioactivity associated with the acetylated product. Although the
sensitivity of the assay depends on the specific radioactivity of the
acetic anhydride used, as low as 0.5 nmol of lysophospholipid in tissue
samples can be readily quantitated. The results obtained from the control
and ischemic canine cardiac tissues by this assay compares favorably with
those obtained by lipid-phosphorus assay. The sensitivity and specificity
of the present procedure allows us and other investigators to assay for
lysophosphatidylcholine content in very small (10 mg wet weight) tissue
samples.
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