J. Lipid Res.
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Journal of Lipid Research, Vol 27, 120-124, Copyright © 1986 by Lipid Research, Inc.

ARTICLES

Short bed-continuous development thin-layer chromatography of glycosphingolipids

WW Young Jr and CA Borgman

The technique of short bed--continuous development chromatography has been utilized to increase the separation of glycosphingolipids on high performance thin-layer chromatography plates. The theoretical goal of increasing separation of bands by decreasing solvent strength was achieved within a practical time span as a result of the high solvent velocities in the short bed tank. Examples are given for increased separation of short and long chain neutral glycolipids, acetylated neutral glycolipids, and gangliosides.
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Copyright © 1986 by the American Society for Biochemistry and Molecular Biology.