Journal of Lipid Research, Vol 27, 1104-1107, Copyright © 1986 by Lipid Research, Inc.
Separation and quantitation of molecular species of plant phosphatidylcholine by high-performance liquid chromatography with flame ionization detection
HA Norman and JB St. John
A method is described for the direct quantitation of phosphatidylcholine
molecular species by reverse phase high-performance liquid chromatography
employing flame ionization detection. The method is shown to be applicable
to plan phosphatidylcholine. The molecular species are separated with a C18
column eluted in an isocratic mode. Detection by a commercially available
flame ionization detector overcomes the problems of detecting underivatized
naturally occurring lipids using ultraviolet detectors, and allows direct
and rapid mass determination of the resolved molecular species. Detection
limits for quantitation are defined.