J. Lipid Res.
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Journal of Lipid Research, Vol 27, 1324-1327, Copyright © 1986 by Lipid Research, Inc.

ARTICLES

Preparative electrophoresis of human apolipoprotein E: an improved method

S Meunier, P Gambert, J Desgres and C Lallemant

Apolipoprotein E was isolated from human very low density lipoproteins by a two-step electrophoretic procedure derived from that of Mendez (1982. Anal. Biochem. 126: 403-408). It included separation in a sodium dodecylsulfate polyacrylamide slab gel, transfer into an agarose gel, and extraction by ultracentrifugation for 30 min. No protein labeling, dialysis, or concentration procedures were needed. The method was fast, showed an excellent protein recovery, and could be suitable as a general method of protein isolation by polyacrylamide gel electrophoresis.
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