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Journal of Lipid Research, Vol 27, 172-176, Copyright © 1986 by Lipid Research, Inc.

ARTICLES

Desorption chemical ionization mass spectrometry of 1-O-alkyl-2-O- acetyl-sn-glycero-3-phosphocholines and analogues

MJ Thomas, M Samuel, RL Wykle, JR Surles and C Piantadosi

Ammonia desorption chemical ionization of ether-linked phospholipids of the type 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine (platelet- activating factors) and a series of analogues revealed a systematic fragmentation pattern that is characteristic for these compounds. The predominant ions included the protonated molecular ion and a series of fragments derived from the molecular ion having the following nominal mass losses: MH-14, MH-42, MH-59, and MH-183. Deuterated ammonia was used to elucidate the nature of several fragments. In addition, desorption chemical ionization was used to quantitate 1-O-hexadecyl-2-O- acetyl-sn-glycero-3-phosphocholine at the nanogram/sample level.
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