Journal of Lipid Research, Vol 27, 215-220, Copyright © 1986 by Lipid Research, Inc.
Determination of ketone body kinetics using a D-(-)-3-hydroxy[4,4,4- 2H3]butyrate tracer
PF Bougneres, EO Balasse, P Ferre and DM Bier
In studies where D-(-)-3-hydroxy[4,4,4-2H3]butyrate is employed as isotopic
tracer in vivo, we have described a selected ion monitoring, gas-liquid
chromatography-mass spectrometry micromethod which measures [2H3] tracer
enrichment in 3-hydroxybutyrate and acetoacetate from 300- microliters
blood samples. For plasma samples in the physiologic range, intra- and
interassay precisions for each ketone averaged better than +/- 1% and +/-
2%, respectively. The use of the method was validated by comparing kinetic
data obtained with the above tracer with simultaneous flux data obtained
with conventional D-(-)-3-hydroxy[3-14C]butyrate tracer in five fasted
rats.
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