Journal of Lipid Research, Vol 27, 377-385, Copyright © 1986 by Lipid Research, Inc.
Apolipophorin III in locusts: purification and characterization
H Chino and M Yazawa
Three molecular species of apolipophorin III were purified from adult
locust hemolymph by gel filtration and ion-exchange chromatography, and
named apo-III-a, apo-III-b, and apo-III-c, respectively. They were
indistinguishable by SDS-polyacrylamide gel electrophoresis,
immunodiffusion, and in amino acid composition; however, they had different
isoelectric points (5.43 for a, 5.11 for b, and 4.98 for c) and, therefore,
could be separated by native- or urea-gel electrophoresis. All three
apo-IIIs were glycoproteins and contained fucose, mannose, and glucosamine.
The total sugar content amounted to about 11% for each of the three
apo-IIIs. The molecular weight of apo- III determined by SDS-polyacrylamide
gel electrophoresis was approximately 20,000, almost equivalent to the
native molecular weight (approximately 19,000) estimated by the
sedimentation-equilibrium method. This indicated that the locust apo-III
exists in hemolymph as a monomeric form. It was demonstrated that a total 9
moles of apo-III (2 moles apo-III-a, 6 moles apo-III-b, and 1 mole
apo-III-c) associate with each mole of lipophorin in response to the action
of locust adipokinetic hormone.
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