Journal of Lipid Research, Vol 27, 457-460, Copyright © 1986 by Lipid Research, Inc.

ARTICLES

Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis

C Gabelli, DG Stark, RE Gregg and HB Brewer Jr

Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus. However, the matrix of this low percentage gel is relatively weak, and one can separate the two forms of apoB in a slab gel apparatus only if one utilizes a gradient polyacrylamide gel or a higher percentage polyacrylamide gel which results in a poorer separation of the protein bands. We have developed an agarose-acrylamide gel electrophoretic method to separate the two major apoB forms. The gel is a mixture of 0.5% agarose and 2% acrylamide. The agarose-acrylamide method is fast, has the advantage of being able to be used on an analytical or preparative scale in a vertical slab gel apparatus, and the gel is of sufficient strength to be used in immunoblotting and/or radioautography.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. M. Noh, K. A. Brayton, W. C. Brown, J. Norimine, G. R. Munske, C. M. Davitt, and G. H. Palmer Composition of the Surface Proteome of Anaplasma marginale and Its Role in Protective Immunity Induced by Outer Membrane Immunization Infect. Immun., May 1, 2008; 76(5): 2219 - 2226. [Abstract] [Full Text] [PDF]

				C. Gabelli, C. Bilato, S. Martini, G. E. Tennyson, L. A. Zech, A. Corsini, M. Albanese, H. B. Brewer, G. Crepaldi, and G. Baggio Homozygous Familial Hypobetalipoproteinemia: Increased LDL Catabolism in Hypobetalipoproteinemia Due to a Truncated Apolipoprotein B Species, Apo B-87Padova Arterioscler. Thromb. Vasc. Biol., September 1, 1996; 16(9): 1189 - 1196. [Abstract] [Full Text]