HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Johnson, F. L. Articles by Rudel, L. L. Search for Related Content PubMed PubMed Citation Articles by Johnson, F. L. Articles by Rudel, L. L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 27, 537-548, Copyright © 1986 by Lipid Research, Inc.

ARTICLES

High density lipoprotein accumulation in perfusates of isolated livers of African green monkeys. Effects of saturated versus polyunsaturated dietary fat

FL Johnson, J Babiak and LL Rudel

To determine whether altered hepatic secretion of HDL is part of the mechanism by which polyunsaturated fat lowers plasma HDL concentration, we have studied HDL secretion in the isolated perfused livers of African green monkeys fed an atherogenic diet containing either safflower oil as the polyunsaturated fat or butter as the saturated fat. During recirculating perfusion with a lipoprotein-free medium, livers from safflower oil-fed animals produced 21% less HDL mass on the average than those from butter-fed animals. Newly secreted hepatic HDL were characterized after their isolation and subfractionation by a combination of agarose column chromatography and density gradient ultracentrifugation. In both diet groups the HDL were heterogeneous in size, morphology, and composition and consisted of discoidal particles ranging in diameter from greater than 200 A to as little as 50 A. Large, discoidal particles that were rich in apoE and apoA-I were separated from small particles that were poor in apoE but rich in apoA- I. All hepatic HDL subfractions contained only small amounts of cholesteryl ester and triglyceride. The hepatic particles resembled in composition and structure the large variety of HDL particles found in the plasma of patients with the familial deficiency of lecithin:cholesterol acyltransferase. Accordingly, perfusate LCAT activity was measured and found to be 2% or less than that in monkey plasma. We conclude that the perfused monkey liver produces a variety of nascent HDL that are relatively unmodified by the post-secretory metabolic events which normally occur in blood plasma in vivo, and that livers of polyunsaturated fat-fed monkeys secrete fewer plasma HDL precursor particles than do those of saturated fat-fed monkeys.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C.-M. Li, J. B. Presley, X. Zhang, N. Dashti, B. H. Chung, N. E. Medeiros, C. Guidry, and C. A. Curcio Retina expresses microsomal triglyceride transfer protein: implications for age-related maculopathy J. Lipid Res., April 1, 2005; 46(4): 628 - 640. [Abstract] [Full Text] [PDF]

				J. W. Furbee Jr., O. Francone, and J. S. Parks In vivo contribution of LCAT to apolipoprotein B lipoprotein cholesteryl esters in LDL receptor and apolipoprotein E knockout mice J. Lipid Res., March 1, 2002; 43(3): 428 - 437. [Abstract] [Full Text] [PDF]

				K. W. Huggins, E. R. Burleson, J. K. Sawyer, K. Kelly, L. L. Rudel, and J. S. Parks Determination of the tissue sites responsible for the catabolism of large high density lipoprotein in the African green monkey J. Lipid Res., March 1, 2000; 41(3): 384 - 394. [Abstract] [Full Text]

				P. L. Colvin, E. Moriguchi, P. H. R. Barrett, J. S. Parks, and L. L. Rudel Small HDL particles containing two apoA-I molecules are precursors in vivo to medium and large HDL particles containing three and four apoA-I molecules in nonhuman primates J. Lipid Res., October 1, 1999; 40(10): 1782 - 1792. [Abstract] [Full Text]

				D. K. Spady, D. M. Kearney, and H. H. Hobbs Polyunsaturated fatty acids up-regulate hepatic scavenger receptor B1 (SR-BI) expression and HDL cholesteryl ester uptake in the hamster J. Lipid Res., August 1, 1999; 40(8): 1384 - 1394. [Abstract] [Full Text]

				M. N. Nanjee, J. E. Doran, P. G. Lerch, and N. E. Miller Acute Effects of Intravenous Infusion of ApoA1/Phosphatidylcholine Discs on Plasma Lipoproteins in Humans Arterioscler. Thromb. Vasc. Biol., April 1, 1999; 19(4): 979 - 989. [Abstract] [Full Text] [PDF]

				K. W. Huggins, L. K. Curtiss, A. K. Gebre, and J. S. Parks Effect of long chain polyunsaturated fatty acids in the sn-2 position of phosphatidylcholine on the interaction with recombinant high density lipoprotein apolipoprotein A-I J. Lipid Res., December 1, 1998; 39(12): 2423 - 2431. [Abstract] [Full Text]

				J. W. Burgess, D. R. Gould, and Y. L. Marcel The HepG2 Extracellular Matrix Contains Separate Heparinase- and Lipid-releasable Pools of ApoE. IMPLICATIONS FOR HEPATIC LIPOPROTEIN METABOLISM J. Biol. Chem., March 6, 1998; 273(10): 5645 - 5654. [Abstract] [Full Text] [PDF]