HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oulton, M. Articles by Faulkner, G. Search for Related Content PubMed PubMed Citation Articles by Oulton, M. Articles by Faulkner, G. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 27, 602-612, Copyright © 1986 by Lipid Research, Inc.

ARTICLES

Quantification of surfactant pool sizes in rabbit lung during perinatal development

M Oulton, M Fraser, M Dolphin, R Yoon and G Faulkner

Methods are presented for the quantitative isolation of surfactants from fetal and newborn rabbit alveolar lavage returns and post-lavaged lung tissue homogenates. The phospholipid content of both fractions progressively increased between 27 days gestation and term (31 days). The tissue-stored fraction increased approximately 16-fold (from 0.48 +/- 0.13 to 7.83 +/- 0.86 mg/g dry lung) and the alveolar fraction more than 30-fold (from 0.08 +/- 0.02 to 2.69 +/- 0.52 mg/g dry lung). Developmental changes in phospholipid composition were also observed. Tissue-stored surfactant was prepared using differential and density gradient centrifugation. Alveolar surfactant was isolated during fetal development as a high-speed pellet following a one-step differential centrifugation. There was little change in the phospholipid content of fetal alveolar lavage supernatant (range 0.12 +/- 0.04 to 0.28 +/- 0.09 mg/g dry lung). By the first postnatal day the phospholipid content of both lavage fractions significantly increased (pellet, 7.51 +/- 1.79; supernatant, 4.01 +/- 1.36 mg/g dry lung) and both were identified as surfactant. This increase in alveolar surfactant was accompanied by an approximately twofold decrease (to 3.81 +/- 1.1 mg/g dry lung) in the tissue-stored fraction. These data provide a quantitative profile of surfactant accumulation and secretion in developing rabbit lung.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. C. Sullivan, S. Orgeig, and C. B. Daniels Control of the development of the pulmonary surfactant system in the saltwater crocodile, Crocodylus porosus Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2002; 283(5): R1164 - R1176. [Abstract] [Full Text] [PDF]

				V. Allen, M. Oulton, D. Stinson, J. MacDonald, and A. Allen Alveolar metabolism of natural vs. synthetic surfactants in preterm newborn rabbits J Appl Physiol, January 1, 2001; 90(1): 198 - 204. [Abstract] [Full Text] [PDF]

				M. Oulton, E. Edwards, and K. Handa Convertase activity in alveolar surfactant and lamellar bodies in fetal, newborn, and adult rabbits J Appl Physiol, January 1, 1999; 86(1): 71 - 77. [Abstract] [Full Text] [PDF]