HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zechner, R. Articles by Kostner, G. M. Search for Related Content PubMed PubMed Citation Articles by Zechner, R. Articles by Kostner, G. M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 27, 681-686, Copyright © 1986 by Lipid Research, Inc.

ARTICLES

Isolation of pure LpB from human serum

R Zechner, R Moser and GM Kostner

Low density lipoproteins (LDL), even after isolation from a narrow density cut and after several washes by preparative ultracentrifugation, are contaminated by 3-5% non-apoB proteins. Incubation of these LDL with artificial triglyceride-rich lipid emulsions (TGRP) removed all contaminating apoC and also, under certain conditions, apoA proteins. TGRP treatment did not, however, change the lipid composition and the flotation behavior of LDL. Residual apoE and albumin, amounting up to 0.5% of the apoB mass, were resistant to removal by TGRP treatment as well as by heparin-Sepharose column chromatography. ApoE and albumin could only be removed by immunoabsorption.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?