Journal of Lipid Research, Vol 27, 781-785, Copyright © 1986 by Lipid Research, Inc.
Transfer of cholesterol and a fluorescent cholesterol analog, 3'- pyrenylmethyl-23,24-dinor-5-cholen-22-oate-3 beta-ol, between human plasma high density lipoproteins
YJ Kao, MC Doody and LC Smith
A fluorescent cholesterol analog, 3'-pyrenylmethyl-23,24-dinor-5-cholen-
22-oate-3 beta-ol (PMCA), has been synthesized as a spectroscopic probe of
cholesterol function. The substrate activity of PMCA, about two- thirds
that of cholesterol, with lecithin:cholesterol acyltransferase indicates
that PMCA is a reasonable cholesterol analog and that the orientation of
the substituted sterol in the phospholipid interface is similar to that of
cholesterol. The fluorescence properties of PMCA are similar to those of
other pyrene-containing compounds that exhibit concentration-dependent
excimer fluorescence. The rate of transfer of [3H]PMCA between HDL is about
six times faster than cholesterol. These results indicate that the analog
will be useful in studies of cholesterol function.
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