Journal of Lipid Research, Vol 27, 901-904, Copyright © 1986 by Lipid Research, Inc.
Enterohepatic circulation in man. A simple method for the determination of duodenal bile acids
RJ Vonk, CM Kneepkens, R Havinga, F Kuipers and CM Bijleveld
A method has been developed for easy sampling of duodenal bile acids. For
this purpose Entero-Test was used, an encapsulated nylon thread originally
used to estimate enteral parasites. This capsule is swallowed by a fasting
subject and one end of the thread is taped at a corner of the month. Four
hours after swallowing the thread, it is withdrawn and bile acids are
eluted with buffer. The solution is applied to a Sep-Pak C18 cartridge to
extract bile acids, which are subsequently analyzed by capillary gas-liquid
chromatography and liquid chromatography. In vitro analyses showed that
there was no preferential binding to the thread of any bile acid and that
binding was pH- independent. A high correlation (r = 0.98) was found
between direct analyses of bile and analyses by Entero-Test after in vitro
incubation. The values obtained by the Entero-Test were similar to those of
duodenal bile simultaneously collected with the normal intubation technique
(r = 0.99). Duodenal bile acid composition showed a daily variation. In 11
healthy volunteers the following bile acid composition of unstimulated
duodenal juice was found (mean +/- SD; %): choleate 44 +/- 12
(glycine/taurine ratio 1.8), chenodeoxycholate: 29 +/- 6 (G/T ratio 2.3);
deoxycholate: 25 +/- 11 (G/T ratio 5.7), lithocholate: 1, ursodeoxycholate:
less than 1. The described technique turned out to be an easily applicable
method for determination of duodenal bile acids in man. This enables
longitudinal studies concerning the factors that determine the bile acid
pool composition and its relevance to various diseases.
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