HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Akiyoshi, T. Articles by Takeuchi, N. Search for Related Content PubMed PubMed Citation Articles by Akiyoshi, T. Articles by Takeuchi, N. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 27, 915-924, Copyright © 1986 by Lipid Research, Inc.

ARTICLES

Cholesterol gallstones in alloxan-diabetic mice

T Akiyoshi, K Uchida, H Takase, Y Nomura and N Takeuchi

Normal and alloxan-diabetic male mice (Crj-ICR) were fed a diet containing 0.5% cholesterol for 5 and 10 weeks, and gallbladder bile was analyzed for cholesterol, phospholipids and bile acids, feces for sterols and bile acids, and plasma and liver for cholesterol, phospholipids, and triglycerides. Normal mice developed no gallstones but the diabetic mice developed cholesterol gallstones with an incidence of 70% by 5 weeks and 80% by 10 weeks after feeding of the cholesterol diet. Diabetic mice fed the ordinary diet also developed stones (23%) by 10 weeks. In the diabetic mice, the gallbladder was enlarged about threefold, and biliary lipid concentration, diet intake, and fecal excretion of sterols and bile acids increased but body weight decreased. Cholic acid and beta-muricholic acid comprised over 40% each of the total biliary bile acids in normal mice, but cholic acid increased to about 80% and beta-muricholic acid decreased to a few percent in the diabetic mice. Fecal excretion of bile acids increased after cholesterol feeding in both normal and diabetic mice, but the increased bile acid in the normal animals was beta-muricholic acid and that in the diabetic mice was deoxycholic acid. The mice that developed gallstones showed a marked increase in biliary cholesterol value and decreases in gallbladder bile and bile acid concentration, but no difference in biliary and fecal bile acid composition, bile acid synthesis, fecal sterols, or plasma and liver lipid levels. Cholesterol absorption was increased in the diabetic mice when examined by plasma 14C/3H ratio and fecal 14C-labeled sterol excretion after a single oral administration of [14C]cholesterol and a simultaneous intravenous injection of [3H]cholesterol. These data led to the conclusion that cholesterol gallstones developed in alloxan-diabetic mice fed excess cholesterol, due to the hyperphagia and the enhancement of cholesterol absorption caused by increases in the synthesis and secretion of cholic acid.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. E. Wu, F. Basso, R. D. Shamburek, M. J. A. Amar, B. Vaisman, G. Szakacs, C. Joyce, T. Tansey, L. Freeman, B. J. Paigen, et al. Hepatic ABCG5 and ABCG8 Overexpression Increases Hepatobiliary Sterol Transport but Does Not Alter Aortic Atherosclerosis in Transgenic Mice J. Biol. Chem., May 28, 2004; 279(22): 22913 - 22925. [Abstract] [Full Text] [PDF]

				D. Q-H. Wang and M. C. Carey Measurement of intestinal cholesterol absorption by plasma and fecal dual-isotope ratio, mass balance, and lymph fistula methods in the mouse: an analysis of direct versus indirect methodologies J. Lipid Res., May 1, 2003; 44(5): 1042 - 1059. [Abstract] [Full Text] [PDF]

				H. Hyogo, S. Roy, B. Paigen, and D. E. Cohen Leptin Promotes Biliary Cholesterol Elimination during Weight Loss in ob/ob Mice by Regulating the Enterohepatic Circulation of Bile Salts J. Biol. Chem., September 6, 2002; 277(37): 34117 - 34124. [Abstract] [Full Text] [PDF]

				D. Q-H. Wang, B. Paigen, and M. C. Carey Genetic factors at the enterocyte level account for variations in intestinal cholesterol absorption efficiency among inbred strains of mice J. Lipid Res., November 1, 2001; 42(11): 1820 - 1830. [Abstract] [Full Text] [PDF]

				J. Férézou, M. Combettes-Souverain, M. Souidi, J. L. Smith, N. Boehler, F. Milliat, E. Eckhardt, G. Blanchard, M. Riottot, C. Sérougne, et al. Cholesterol, bile acid, and lipoprotein metabolism in two strains of hamster, one resistant, the other sensitive (LPN) to sucrose-induced cholelithiasis J. Lipid Res., December 1, 2000; 41(12): 2042 - 2054. [Abstract] [Full Text]

				D. Q-H. Wang, F. Lammert, B. Paigen, and M. C. Carey Phenotypic characterization of Lith genes that determine susceptibility to cholesterol cholelithiasis in inbred mice: pathophysiology of biliary lipid secretion J. Lipid Res., November 1, 1999; 40(11): 2066 - 2079. [Abstract] [Full Text]

				D. Q.-H. Wang, F. Lammert, D. E. Cohen, B. Paigen, and M. C. Carey Cholic acid aids absorption, biliary secretion, and phase transitions of cholesterol in murine cholelithogenesis Am J Physiol Gastrointest Liver Physiol, March 1, 1999; 276(3): G751 - G760. [Abstract] [Full Text] [PDF]

				H. Wittenburg, F. Lammert, D. Q.-H. Wang, G. A. Churchill, R. Li, G. Bouchard, M. C. Carey, and B. Paigen Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice Physiol Genomics, February 11, 2002; 8(1): 67 - 77. [Abstract] [Full Text] [PDF]