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Journal of Lipid Research, Vol 28, 1216-1224, Copyright © 1987 by Lipid Research, Inc.
JM Ordovas, JP Peterson, P Santaniello, JS Cohn, PW Wilson and EJ Schaefer
A noncompetitive enzyme-linked immunosorbent assay (ELISA) has been
developed for measuring total plasma apolipoprotein (apo) B using affinity
purified polyclonal and monoclonal antibodies. Microtiter plates from
different manufacturers were tested with regard to their IgG binding
characteristics; only one plate yielded consistent coefficients of
variation of less than 5%. The optimal plasma dilution in this assay was
1:3000. IgG anti-apoB antisera conjugated to alkaline phosphatase was used
as a second antibody. p-Nitrophenyl phosphate was utilized as substrate for
color development, and the absorbance (410 nm) was read utilizing an ELISA
reader interfaced with a microcomputer for data processing. Plasma apoB
levels in plasma have been determined in 1115 male and female participants
in the Framingham Offspring Study. Mean (+/- SD) plasma concentrations were
89 +/- 28 mg/dl. Significant age and sex related differences in apoB levels
were noted.
ARTICLES
Enzyme-linked immunosorbent assay for human plasma apolipoprotein B
USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA.
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