J. Lipid Res.
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Journal of Lipid Research, Vol 28, 1370-1372, Copyright © 1987 by Lipid Research, Inc.

ARTICLES

Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha- hydroxysteroid dehydrogenase

JM Little, P Zimniak, A Radominska and R Lester
Department of Internal Medicine, University of Texas Medical School at Houston 77225.

The reaction of the 6-hydroxylated bile acid, hyodeoxycholic acid, and its 6-O-glucuronide conjugate with 3 alpha-hydroxysteroid dehydrogenase was examined. A standard end-point assay and determination of the initial rates of reaction showed only minimal activity of the enzyme toward hyodeoxycholate-6-glucuronide in spite of the presence of a free 3 alpha-hydroxyl group. It was established that 6-hydroxylation itself did not significantly affect the enzyme reaction. It is concluded that the 6-glucuronide either blocks or hinders enzyme access to the 3- hydroxyl group.
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Copyright © 1987 by the American Society for Biochemistry and Molecular Biology.