Journal of Lipid Research, Vol 28, 1377-1383, Copyright © 1987 by Lipid Research, Inc.
ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
EC Mandon, IN de Gomez Dumm, MJ de Alaniz, CA Marra and RR Brenner
Instituto de Investigaciones Bioquimicas de La Plata, CONICET-UNLP, La Plata, Argentina.
The in vivo and in vitro effect of ACTH on the biosynthesis of
polyunsaturated fatty acid of rat adrenal gland and liver was studied. The
administration of ACTH to intact rats produced a significant decrease in
the conversion of [1-14C]linoleic acid to gamma-linolenic acid,
[1-14C]alpha-linolenic acid to octadeca-6,9,12,15-tetraenoic acid, and
[1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid in liver and
adrenal microsomes. Isolated adrenocortical cells and hepatocytes obtained
from animals treated with ACTH showed a decrease in the incorporation and
desaturation of exogenous [1-14C]eicosa- 8,11,14-trienoic acid. The
addition of ACTH to the incubation medium of adrenocortical cells and
hepatocytes isolated from untreated rats also caused a decrease in delta 5
desaturation activity. The effect of ACTH on adrenal and liver desaturases
could be produced as a consequence of the release of glucocorticoids,
already measured in the experiments. However, the in vitro experiments
carried out with hepatocytes isolated from untreated rats, where
corticosterone was absent, indicated that ACTH can depress delta 5
desaturation per se.
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