Journal of Lipid Research, Vol 28, 119-129, Copyright © 1987 by Lipid Research, Inc.
Alterations in plasma lipoproteins and apolipoproteins in experimental allergic encephalomyelitis
VG Shore, ME Smith, V Perret and MA Laskaris
Plasma lipoproteins were investigated during the active clinical phase of
experimental allergic encephalomyelitis (EAE), a demyelinating disease of
the central nervous system. Three groups of Lewis rats were compared:
untreated controls, Freund's adjuvant-treated controls (FAC), and rats
receiving one injection of myelin in Freund's adjuvant. After onset of
clinical symptoms, 12 and 16 days after injection, there were higher
concentrations of cholesterol and low and high density lipoproteins (LDL
and HDL) in EAE plasma. The increase was due to apoE- containing HDL1 and
HDL, according to density, particle size, and apolipoprotein compositions
of isolated lipoproteins and immunoblots of whole plasmas after gradient
gel electrophoresis. In EAE, the cholesterol-to-apoprotein ratio was
increased and the low density lipoprotein distribution profile was shifted
toward lower density. The Freund's adjuvant-treated control rats showed
some changes qualitatively similar to those of EAE, albeit far smaller in
magnitude. Changes in LDL in EAE might be related in part to lowered plasma
very low density lipoproteins (VLDL); however, weight loss in control
animals did not increase plasma cholesterol or apoE relative to apoA-I.
Lesions in the central nervous system and/or activation of macrophages
might be causally related to the large increase in plasma apoE. The major
changes in apoE-containing lipoproteins are undoubtedly significant for the
altered immune function in EAE.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?