Journal of Lipid Research, Vol 28, 138-143, Copyright © 1987 by Lipid Research, Inc.
Sidedness of ceramide-phosphoethanolamine synthesis on rat liver and brain microsomal membranes
M Malgat, A Maurice and J Baraud
Phosphatidylethanolamine:ceramide-ethanolamine-phosphotransferase catalyzes
the synthesis of ceramide-phosphoethanolamine, a sphingomyelin analogue.
Its localization was studied in rat liver and brain microsomes. After
testing the integrity and the sidedness of microsomal vesicles, trypsin
treatment of intact or deoxycholate- disrupted microsomes made it possible
to conclude that both the transferase and the ceramide-phosphoethanolamine
are located in the cisternal leaflet of the membrane bilayer. Using
trinitrobenzenesulfonic acid as a probe, no trace of newly synthesized
ceramide-phosphoethanolamine was detectable on the cytoplasmic side of the
microsomes.