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J. Lipid Res.
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Journal of Lipid Research, Vol 28, 138-143, Copyright © 1987 by Lipid Research, Inc.


ARTICLES

Sidedness of ceramide-phosphoethanolamine synthesis on rat liver and brain microsomal membranes

M Malgat, A Maurice and J Baraud

Phosphatidylethanolamine:ceramide-ethanolamine-phosphotransferase catalyzes the synthesis of ceramide-phosphoethanolamine, a sphingomyelin analogue. Its localization was studied in rat liver and brain microsomes. After testing the integrity and the sidedness of microsomal vesicles, trypsin treatment of intact or deoxycholate- disrupted microsomes made it possible to conclude that both the transferase and the ceramide-phosphoethanolamine are located in the cisternal leaflet of the membrane bilayer. Using trinitrobenzenesulfonic acid as a probe, no trace of newly synthesized ceramide-phosphoethanolamine was detectable on the cytoplasmic side of the microsomes.
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