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Journal of Lipid Research, Vol 28, 414-422, Copyright © 1987 by Lipid Research, Inc.
M Beylot, C Martin, B Beaufrere, JP Riou and R Mornex
Using deuterium-labeled glycerol as tracer and gas-liquid
chromatography-mass spectrometry techniques for the determination of
isotopic enrichment, we have developed a simple and ethically acceptable
method of determining glycerol appearance rate in humans under steady-state
and nonsteady-state conditions. In normal subjects, the appearance rate of
glycerol in the post-absorptive state was 2.22 +/- 0.20 mumol X kg-1 X
min-1, a value in agreement with those reported in studies with
radioactively labeled tracers. The ratio nonesterified fatty acid (NEFA)
appearance rate/glycerol appearance rate ranged from 1.95 to 3.40. In
insulin-dependent diabetic patients with a mild degree of metabolic
control, the appearance rate of glycerol was 2.48 +/- 0.29 mumol X kg-1 X
min-1. The volume of distribution of glycerol, determined by the bolus
injection technique, was (mean) 0.306 l X kg-1 in normal subjects and 0.308
l X kg-1 in insulin-independent diabetic patients. To evaluate the
usefulness of the method for determination of glycerol kinetics in
nonsteady-state conditions, we infused six normal subjects with natural
glycerol and calculated the isotopically determined glycerol appearance
rate using a single compartment model (volume of distribution 0.31 l X
kg-1). During these tests, the expected glycerol appearance rates were
successively 5.03 +/- 0.33, 7.48 +/- 0.39, 9.94 +/- 0.34, 7.48 +/- 0.39,
and 5.03 +/- 0.33 mumol +/- kg-1 X min-1, whereas the corresponding
isotopically determined appearance rates were 4.62 +/- 0.45, 6.95 +/- 0.56,
10.85 +/- 0.51, 7.35 +/- 0.34, and 5.28 +/- 0.12 mumol X kg-1 X
min-1.(ABSTRACT TRUNCATED AT 250 WORDS)
ARTICLES
Determination of steady state and nonsteady-state glycerol kinetics in humans using deuterium-labeled tracer
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