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Journal of Lipid Research, Vol 28, 455-463, Copyright © 1987 by Lipid Research, Inc.
LM Havekes, P de Knijff, U Beisiegel, J Havinga, M Smit and E Klasen
A new method for the apolipoprotein E phenotyping has been developed. The
method is based on isoelectric focusing of either delipidated or
guanidine-HC1-treated serum or plasma in a horizontal slab gel system
followed by immunoblotting using either polyclonal or monoclonal anti-
apolipoprotein E antibodies as first antibody. Apolipoprotein E phenotyping
with this method in 200 serum samples that had been stored at -20 degrees C
for more than one year gave exactly the same results as obtained with the
conventional method based on isoelectric focusing of delipidated very low
density lipoproteins isolated from fresh serum followed by protein
staining. Compared with the conventional method, the present method is less
laborious because ultracentrifugation to isolate VLDL is not needed; it is
suitable for large scale screening purposes; it needs only a few
microliters of serum or plasma, and can easily be performed with samples
with low concentrations of apolipoprotein E.
ARTICLES
A rapid micromethod for apolipoprotein E phenotyping directly in serum
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