| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 28, 495-509, Copyright © 1987 by Lipid Research, Inc.
H Esterbauer, G Jurgens, O Quehenberger and E Koller
The alteration of structural and biological properties of human plasma low
density lipoprotein (LDL) exposed to oxidative conditions is in part
ascribed to lipid peroxidation. The objective of this investigation was to
measure quantitatively several parameters in oxidizing LDL indicative for
lipid peroxidation. Exposure of freshly prepared EDTA-free LDL to an
oxygen-saturated buffer led to a complete depletion of alpha- and
gamma-tocopherol within 6 hr, thereafter lipid peroxidation commenced as
indicated by the kinetics of the loss of linoleic (18:2) and arachidonic
(20:4) acids, the formation of aldehydic lipid peroxidation products and
fluorescent apoB. Within 24 hr of oxidation, on average 79 nmol of 18:2
(initial 345) and 12.8 nmol of 20.4 (initial 25.6) were oxidized per mg of
LDL and the sample contained in total 7.1 nmol of aldehydes with the
following molar distribution: 36.6% malonaldehyde, 25% hexanal, 8.9%
propanal, 8.2% 4- hydroxynonenal, 7.6% butanal, 4.1% 2.4-heptadienal, 3.4%
pentanal, 3.4% 4-hydroxyhexenal, and 2.5% 4-hydroxyoctenal. Malonaldehyde
was predominantly (93%) in the aqueous phase, whereas the other aldehydes
remained mostly (34-98%) within the LDL particle, where the total aldehyde
concentration was in the range of 12 mM. Oxidized LDL exhibited a 1.6-fold
enhanced electrophoretic mobility. Similarily, native LDL incubated for 5
hr with aldehydes showed increased electrophoretic mobility. At equal
concentrations (5 mM) 4- hydroxynonenal was most effective, followed by
2,4-heptadienal, hexanal, and malonaldehyde. This study reports for the
first time the rate and extent of the change of LDL constituents occurring
during lipid peroxidation.
ARTICLES
Autoxidation of human low density lipoprotein: loss of polyunsaturated fatty acids and vitamin E and generation of aldehydes
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. J.A. Korporaal, M. Van Eck, J. Adelmeijer, M. Ijsseldijk, R. Out, T. Lisman, P. J. Lenting, T. J.C. Van Berkel, and J.-W. N. Akkerman Platelet Activation by Oxidized Low Density Lipoprotein Is Mediated by Cd36 and Scavenger Receptor-A Arterioscler. Thromb. Vasc. Biol., November 1, 2007; 27(11): 2476 - 2483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Carnevale, P. Pignatelli, L. Lenti, B. Buchetti, V. Sanguigni, S. Di Santo, and F. Violi LDL are oxidatively modified by platelets via GP91phox and accumulate in human monocytes FASEB J, March 1, 2007; 21(3): 927 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Pfrommer, W. Erl, and P. C. Weber Docosahexaenoic acid induces ciap1 mRNA and protects human endothelial cells from stress-induced apoptosis Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2178 - H2186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Stocker and J. F. Keaney Jr. Role of Oxidative Modifications in Atherosclerosis Physiol Rev, October 1, 2004; 84(4): 1381 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Traverso, S. Menini, E. P. Maineri, S. Patriarca, P. Odetti, D. Cottalasso, U. M. Marinari, and M. A. Pronzato Malondialdehyde, a Lipoperoxidation-Derived Aldehyde, Can Bring About Secondary Oxidative Damage To Proteins J. Gerontol. A Biol. Sci. Med. Sci., September 1, 2004; 59(9): B890 - B895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Keightley, L. Shang, and M. Kinter Proteomic Analysis of Oxidative Stress-resistant Cells: A SPECIFIC ROLE FOR ALDOSE REDUCTASE OVEREXPRESSION IN CYTOPROTECTION Mol. Cell. Proteomics, February 1, 2004; 3(2): 167 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ohgami, K. Shiratori, S. Kotake, T. Nishida, N. Mizuki, K. Yazawa, and S. Ohno Effects of Astaxanthin on Lipopolysaccharide-Induced Inflammation In Vitro and In Vivo Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2694 - 2701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kobayashi, M. Kishi, T. Atsumi, M. L. Bertolaccini, H. Makino, N. Sakairi, I. Yamamoto, T. Yasuda, M. A. Khamashta, G. R. V. Hughes, et al. Circulating oxidized LDL forms complexes with {beta}2-glycoprotein I: implication as an atherogenic autoantigen J. Lipid Res., April 1, 2003; 44(4): 716 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pankhurst, X. L. Wang, D. E. Wilcken, G. Baernthaler, U. Panzenbock, M. Raftery, and R. Stocker Characterization of specifically oxidized apolipoproteins in mildly oxidized high density lipoprotein J. Lipid Res., February 1, 2003; 44(2): 349 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cnop, J. C. Hannaert, A. Y. Grupping, and D. G. Pipeleers Low Density Lipoprotein Can Cause Death of Islet {beta}-Cells by Its Cellular Uptake and Oxidative Modification Endocrinology, September 1, 2002; 143(9): 3449 - 3453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huber, H. Boechzelt, B. Karten, M. Surboeck, V. N. Bochkov, B. R. Binder, W. Sattler, and N. Leitinger Oxidized Cholesteryl Linoleates Stimulate Endothelial Cells to Bind Monocytes via the Extracellular Signal-Regulated Kinase 1/2 Pathway Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 581 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chancharme, P. Therond, F. Nigon, S. Zarev, A. Mallet, E. Bruckert, and M. J. Chapman LDL particle subclasses in hypercholesterolemia: molecular determinants of reduced lipid hydroperoxide stability J. Lipid Res., March 1, 2002; 43(3): 453 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Friedman, S. Horkko, D. Steinberg, J. L. Witztum, and E. A. Dennis Correlation of Antiphospholipid Antibody Recognition with the Structure of Synthetic Oxidized Phospholipids. IMPORTANCE OF SCHIFF BASE FORMATION AND ALDOL CONDENSATION J. Biol. Chem., February 22, 2002; 277(9): 7010 - 7020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Upston, X. Niu, A. J. Brown, R. Mashima, H. Wang, R. Senthilmohan, A. J. Kettle, R. T. Dean, and R. Stocker Disease Stage-Dependent Accumulation of Lipid and Protein Oxidation Products in Human Atherosclerosis Am. J. Pathol., February 1, 2002; 160(2): 701 - 710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kamido, H. Eguchi, H. Ikeda, T. Imaizumi, K. Yamana, K. Hartvigsen, A. Ravandi, and A. Kuksis Core aldehydes of alkyl glycerophosphocholines in atheroma induce platelet aggregation and inhibit endothelium-dependent arterial relaxation J. Lipid Res., January 1, 2002; 43(1): 158 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Perrin-Cocon, F. Coutant, S. Agaugue, S. Deforges, P. Andre, and V. Lotteau Oxidized Low-Density Lipoprotein Promotes Mature Dendritic Cell Transition from Differentiating Monocyte J. Immunol., October 1, 2001; 167(7): 3785 - 3791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hattori, S. Hattori, and K. Kasai 4-Hydroxynonenal Prevents NO Production in Vascular Smooth Muscle Cells by Inhibiting Nuclear Factor-{{kappa}}B-Dependent Transcriptional Activation of Inducible NO Synthase Arterioscler. Thromb. Vasc. Biol., July 1, 2001; 21(7): 1179 - 1183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Haidari, E. Javadi, M. Kadkhodaee, and A. Sanati Enhanced Susceptibility to Oxidation and Diminished Vitamin E Content of LDL from Patients with Stable Coronary Artery Disease Clin. Chem., July 1, 2001; 47(7): 1234 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kobayashi, E. Matsuura, Q. Liu, J.-i. Furukawa, K. Kaihara, J. Inagaki, T. Atsumi, N. Sakairi, T. Yasuda, D. R. Voelker, et al. A specific ligand for {beta}2-glycoprotein I mediates autoantibody-dependent uptake of oxidized low density lipoprotein by macrophages J. Lipid Res., May 1, 2001; 42(5): 697 - 709. [Abstract] [Full Text]
|
|
|
|
|
|
A. Roland, R. A. Patterson, and D. S. Leake Measurement of Copper-Binding Sites on Low Density Lipoprotein Arterioscler. Thromb. Vasc. Biol., April 1, 2001; 21(4): 594 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Carr, B.-Z. Zhu, and B. Frei Potential Antiatherogenic Mechanisms of Ascorbate (Vitamin C) and {alpha}-Tocopherol (Vitamin E) Circ. Res., September 1, 2000; 87(5): 349 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kusmic, E. Picano, C. L. Busceti, C. Petersen, and R. Barsacchi The antioxidant drug dipyridamole spares the vitamin E and thiols in red blood cells after oxidative stress Cardiovasc Res, August 18, 2000; 47(3): 510 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Volf, E. Bielek, T. Moeslinger, F. Koller, and E. Koller Modification of Protein Moiety of Human Low Density Lipoprotein by Hypochlorite Generates Strong Platelet Agonist Arterioscler. Thromb. Vasc. Biol., August 1, 2000; 20(8): 2011 - 2018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-T. Lee, L.-Y. Chiang, W.-J. Chen, and H.-C. Hsu Water-Soluble Hexasulfobutyl[60]fullerene Inhibit Low-Density Lipoprotein Oxidation in Aqueous and Lipophilic Phases Experimental Biology and Medicine, June 1, 2000; 224(2): 69 - 75. [Abstract] [Full Text]
|
|
|
|
|
|
K. L. Gillotte, S. Hörkkö, J. L. Witztum, and D. Steinberg Oxidized phospholipids, linked to apolipoprotein B of oxidized LDL, are ligands for macrophage scavenger receptors J. Lipid Res., May 1, 2000; 41(5): 824 - 833. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Lepage, F. Nigon, D. Bonnefont-Rousselot, U. Assogba, S. Goulinet, L. Chancharme, J. Delattre, E. Bruckert, and M. J. Chapman Oxidizability of Atherogenic Low-Density Lipoprotein Subspecies in Severe Familial Hypercholesterolemia: Impact of Long-Term Low-Density Lipoprotein Apheresis Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 2000; 5(2): 87 - 103. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Galle and K. Heermeier Angiotensin II and oxidized LDL: an unholy alliance creating oxidative stress Nephrol. Dial. Transplant., November 1, 1999; 14(11): 2585 - 2589. [Full Text] [PDF]
|
|
|
|
 |
|
 M. ANDO, T. SANAKA, and H. NIHEI Eicosapentanoic Acid Reduces Plasma Levels of Remnant Lipoproteins and Prevents in Vivo Peroxidation of LDL in Dialysis Patients J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2177 - 2184. [Abstract] [Full Text]
|
|
|
|
|
|
M. Lougheed, E. D. W. Moore, D. R. L. Scriven, and U. P. Steinbrecher Uptake of Oxidized LDL by Macrophages Differs From That of Acetyl LDL and Leads to Expansion of an Acidic Endolysosomal Compartment Arterioscler. Thromb. Vasc. Biol., August 1, 1999; 19(8): 1881 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Doi, K. Kugiyama, M. Ohgushi, S. Sugiyama, T. Matsumura, Y. Ohta, H. Oka, N. Ogata, A. Hirata, Y. Yamamoto, et al. Membrane Active Lipids in Remnant Lipoproteins Cause Impairment of Endothelium-Dependent Vasorelaxation Arterioscler. Thromb. Vasc. Biol., August 1, 1999; 19(8): 1918 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Salomon, W. Sha, C. Brame, K. Kaur, G. Subbanagounder, J. O'Neil, H. F. Hoff, and L. J. Roberts II Protein Adducts of Iso[4]levuglandin E2, a Product of the Isoprostane Pathway, in Oxidized Low Density Lipoprotein J. Biol. Chem., July 16, 1999; 274(29): 20271 - 20280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Niu, V. Zammit, J. M. Upston, R. T. Dean, and R. Stocker Coexistence of Oxidized Lipids and {alpha}-Tocopherol in All Lipoprotein Density Fractions Isolated From Advanced Human Atherosclerotic Plaques Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1708 - 1718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Letters, P. K. Witting, J. K. Christison, A. W. Eriksson, K. Pettersson, and R. Stocker Time-dependent changes to lipids and antioxidants in plasma and aortas of apolipoprotein E knockout mice J. Lipid Res., June 1, 1999; 40(6): 1104 - 1112. [Abstract] [Full Text]
|
|
|
|
 |
|
 M.-L. Liu, R. Bergholm, S. Makimattila, S. Lahdenpera, M. Valkonen, H. Hilden, H. Yki-Jarvinen, and M.-R. Taskinen A marathon run increases the susceptibility of LDL to oxidation in vitro and modifies plasma antioxidants Am J Physiol Endocrinol Metab, June 1, 1999; 276(6): E1083 - E1091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. DESRUMAUX, V. DECKERT, A. ATHIAS, D. MASSON, G. LIZARD, V. PALLEAU, P. GAMBERT, and L. LAGROST Plasma phospholipid transfer protein prevents vascular endothelium dysfunction by delivering {alpha}-tocopherol to endothelial cells FASEB J, May 1, 1999; 13(8): 883 - 892. [Abstract] [Full Text]
|
|
|
|
|
|
S. Page, C. Fischer, B. Baumgartner, M. Haas, U. Kreusel, G. Loidl, M. Hayn, H. W. L. Ziegler-Heitbrock, D. Neumeier, and K. Brand 4-Hydroxynonenal Prevents NF-{kappa}B Activation and Tumor Necrosis Factor Expression by Inhibiting I{kappa}B Phosphorylation and Subsequent Proteolysis J. Biol. Chem., April 23, 1999; 274(17): 11611 - 11618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. van Reyk, W. Jessup, and R. T. Dean Prooxidant and Antioxidant Activities of Macrophages in Metal-Mediated LDL Oxidation : The Importance of Metal Sequestration Arterioscler. Thromb. Vasc. Biol., April 1, 1999; 19(4): 1119 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chancharme, P. Therond, F. Nigon, S. Lepage, M. Couturier, and M. J. Chapman Cholesteryl Ester Hydroperoxide Lability Is a Key Feature of the Oxidative Susceptibility of Small, Dense LDL Arterioscler. Thromb. Vasc. Biol., March 1, 1999; 19(3): 810 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xing, J. Baffic, and C. P. Sparrow LDL oxidation by activated monocytes: characterization of the oxidized LDL and requirement for transition metal ions J. Lipid Res., November 1, 1998; 39(11): 2201 - 2208. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Holvoet, J. Vanhaecke, S. Janssens, F. Van de Werf, and D. Collen Oxidized LDL and Malondialdehyde-Modified LDL in Patients With Acute Coronary Syndromes and Stable Coronary Artery Disease Circulation, October 13, 1998; 98(15): 1487 - 1494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Karten, H. Boechzelt, P. M. Abuja, M. Mittelbach, K. Oettl, and W. Sattler Femtomole analysis of 9-oxononanoyl cholesterol by high performance liquid chromatography J. Lipid Res., July 1, 1998; 39(7): 1508 - 1519. [Abstract] [Full Text]
|
|
|
|
|
|
B. Chappey, B. Beyssen, E. Foos, F. Ledru, J. L. Guermonprez, J. C. Gaux, and I. Myara Sialic Acid Content of LDL in Coronary Artery Disease: No Evidence of Desialylation in Subjects With Coronary Stenosis and Increased Levels in Subjects With Extensive Atherosclerosis and Acute Myocardial Infarction : Relation Between Desialylation and In Vitro Peroxidation Arterioscler. Thromb. Vasc. Biol., June 1, 1998; 18(6): 876 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Terpstra, D. A. Bird, and D. Steinberg Evidence that the lipid moiety of oxidized low density lipoprotein plays a role in its interaction with macrophage receptors PNAS, February 17, 1998; 95(4): 1806 - 1811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Steinberg Low Density Lipoprotein Oxidation and Its Pathobiological Significance J. Biol. Chem., August 22, 1997; 272(34): 20963 - 20966. [Full Text] [PDF]
|
|
|
|
|
|
M. Lougheed, C. M. Lum, W. Ling, H. Suzuki, T. Kodama, and U. Steinbrecher High Affinity Saturable Uptake of Oxidized Low Density Lipoprotein by Macrophages from Mice Lacking the Scavenger Receptor Class A Type I/II J. Biol. Chem., May 16, 1997; 272(20): 12938 - 12944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Frostegard, Y. H. Huang, J. Ronnelid, and L. Schafer-Elinder Platelet-Activating Factor and Oxidized LDL Induce Immune Activation by a Common Mechanism Arterioscler. Thromb. Vasc. Biol., May 1, 1997; 17(5): 963 - 968. [Abstract] [Full Text]
|
|
|
|
|
|
S. Goulinet and M. J. Chapman Plasma LDL and HDL Subspecies Are Heterogenous in Particle Content of Tocopherols and Oxygenated and Hydrocarbon Carotenoids: Relevance to Oxidative Resistance and Atherogenesis Arterioscler. Thromb. Vasc. Biol., April 1, 1997; 17(4): 786 - 796. [Abstract] [Full Text]
|
|
|
|
|
|
M. S. Bolgar, C.-Y. Yang, and S. J. Gaskell First Direct Evidence for Lipid/Protein Conjugation in Oxidized Human Low Density Lipoprotein J. Biol. Chem., November 8, 1996; 271(45): 27999 - 28001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Demuth, I. Myara, B. Chappey, B. Vedie, M. A. Pech-Amsellem, M. E. Haberland, and N. Moatti A Cytotoxic Electronegative LDL Subfraction Is Present in Human Plasma Arterioscler. Thromb. Vasc. Biol., June 1, 1996; 16(6): 773 - 783. [Abstract] [Full Text]
|
|
|
|
|
|
A. Sevanian, J. Hwang, H. Hodis, G. Cazzolato, P. Avogaro, and G. Bittolo-Bon Contribution of an In Vivo Oxidized LDL to LDL Oxidation and Its Association With Dense LDL Subpopulations Arterioscler. Thromb. Vasc. Biol., June 1, 1996; 16(6): 784 - 793. [Abstract] [Full Text]
|
|
|
|
|
|
S. R. Thomas, J. Neuzil, and R. Stocker Cosupplementation With Coenzyme Q Prevents the Prooxidant Effect of {alpha}-Tocopherol and Increases the Resistance of LDL to Transition Metal–Dependent Oxidation Initiation Arterioscler. Thromb. Vasc. Biol., May 1, 1996; 16(5): 687 - 696. [Abstract] [Full Text]
|
|
|
|
|
|
L.Y. Chen, P. Mehta, and J.L. Mehta Oxidized LDL Decreases L-Arginine Uptake and Nitric Oxide Synthase Protein Expression in Human Platelets : Relevance of the Effect of Oxidized LDL on Platelet Function Circulation, May 1, 1996; 93(9): 1740 - 1746. [Abstract] [Full Text]
|
|
|
|
|
|
M. R. McCall, J. Y. Tang, J. K. Bielicki, and T. M. Forte Inhibition of Lecithin-Cholesterol Acyltransferase and Modification of HDL Apolipoproteins by Aldehydes Arterioscler. Thromb. Vasc. Biol., October 1, 1995; 15(10): 1599 - 1606. [Abstract] [Full Text]
|
|
|
|
|
|
A. Hammer, G. Kager, G. Dohr, H. Rabl, I. Ghassempur, and G. Jurgens Generation, Characterization, and Histochemical Application of Monoclonal Antibodies Selectively Recognizing Oxidatively Modified ApoB-Containing Serum Lipoproteins Arterioscler. Thromb. Vasc. Biol., May 1, 1995; 15(5): 704 - 713. [Abstract] [Full Text]
|
|
|
|
|
|
V. W. Bowry, D. Mohr, J. Cleary, and R. Stocker Prevention of Tocopherol-mediated Peroxidation in Ubiquinol-10-free Human Low Density Lipoprotein J. Biol. Chem., March 17, 1995; 270(11): 5756 - 5763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M.G. Princen, W. van Duyvenvoorde, R. Buytenhek, A. van der Laarse, G. van Poppel, J. A. G. Leuven, and V. W.M. van Hinsbergh Supplementation With Low Doses of Vitamin E Protects LDL From Lipid Peroxidation in Men and Women Arterioscler. Thromb. Vasc. Biol., March 1, 1995; 15(3): 325 - 333. [Abstract] [Full Text]
|
|
|
|
|
|
J. Boren, A. Lookene, E. Makoveichuk, S. Xiang, M. Gustafsson, H. Liu, P. Talmud, and G. Olivecrona Binding of Low Density Lipoproteins to Lipoprotein Lipase Is Dependent on Lipids but Not on Apolipoprotein B J. Biol. Chem., July 13, 2001; 276(29): 26916 - 26922. [Abstract] [Full Text] [PDF]
|
|
