Journal of Lipid Research, Vol 28, 667-672, Copyright © 1987 by Lipid Research, Inc.
Biosynthesis of apolipophorin-III by the fat body in locusts
S Izumi, K Yamasaki, S Tomino and H Chino
Biosynthesis of locust apolipophorin-III (apo-III) was studied in vitro.
Gel electrophoresis and immunoblotting analyses of the locust hemolymph
demonstrated that apo-III first appears in the hemolymph on the day 3 of
the adult stage after the final molt and its hemolymph concentration
increases thereafter. When incubated in vitro in a medium containing
radioactive amino acid, the fat body cells synthesized the radiolabeled
apo-III and released it into the medium. The developmental change in the
apo-III synthesizing activity in the fat body reflected that of the apo-III
concentration in the hemolymph. RNA isolated from the adult fat body
directed the synthesis of apo-III as a major translation product in a
cell-free system. These results indicate that the fat body is the tissue
responsible for the synthesis of the locust apo-III, and biosynthesis of
apo-III is developmentally regulated at the level of mRNA in accordance
with the flight activity of the locust.
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