Journal of Lipid Research, Vol 28, 856-863, Copyright © 1987 by Lipid Research, Inc.
7-Methyl bile acids: 7 beta-methyl-cholic acid inhibits bacterial 7- dehydroxylation of cholic acid and chenodeoxycholic acid in the hamster
S Kuroki, EH Mosbach, BI Cohen, RJ Stenger and CK McSherry
The effect of dietary 7 beta-methyl-cholic acid [0.075% in rodent chow (6.4
mg/animal per day)] on cholesterol and bile acid metabolism was studied and
compared with that of cholic acid in the hamster. Following oral
administration of 7 beta-methyl-cholic acid for 3 weeks, the
glycine-conjugated bile acid analog became a major constituent of
gallbladder bile. Biliary cholic acid concentration decreased
significantly, while that of chenodeoxycholic acid remained unchanged.
Serum and liver cholesterol levels were increased by dietary 7 beta-
methyl-cholic acid and by cholic acid. Hepatic microsomal HMG-CoA reductase
activity was inhibited (30% of the control value) by both bile acids;
cholesterol 7 alpha-hydroxylase activity was not affected. In chow controls
and cholic acid-fed animals, bacterial 7- dehydroxylation of
[14C]chenodeoxycholic acid and [14C]cholic acid was nearly complete. In
contrast, dietary 7 beta-methyl-cholic acid effectively prevented the
7-dehydroxylation of the two primary bile acids. These results show that
dietary 7 beta-methyl-cholic acid is preserved in the enterohepatic
circulation and has an effect on serum and liver cholesterol concentrations
similar to those produced by the naturally occurring cholic acid. 7
beta-Methyl-cholic acid is an efficient inhibitor of the bacterial
7-dehydroxylation of the primary bile acids in the hamster.
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