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Journal of Lipid Research, Vol 28, 873-877, Copyright © 1987 by Lipid Research, Inc.

ARTICLES

A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides

JC Khoo, E Miller and DI Goldberg

An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles.
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