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Journal of Lipid Research, Vol 28, 1013-1015, Copyright © 1987 by Lipid Research, Inc.

ARTICLES

Separation of methylated free bile acids from their taurine and methyl glycine conjugates by thin-layer chromatography

MJ Bolt
Department of Medicine, University of Chicago, IL 60637.

Class separation of methylated free bile acids from bile acids conjugated with taurine and methylglycine was accomplished using a solvent system of 2,2,4-trimethylpentane-absolute ethanol 10:1 (v/v). By developing a silica thin-layer plate two times with solvent in a Brinkmann sandwich tank, the difficult resolution between methyl cholate and methyl glycolithocholate was achieved. Evidence is presented that this separation system may be useful as a preparative step in the analysis of bile acids by gas-liquid chromatography or high pressure liquid chromatography.--Bolt, M. J. G. Separation of methylated free bile acids from their taurine and methyl glycine conjugates by thin-layer chromatography.
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