HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smith, B. F. Search for Related Content PubMed PubMed Citation Articles by Smith, B. F. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 28, 1088-1097, Copyright © 1987 by Lipid Research, Inc.

ARTICLES

Human gallbladder mucin binds biliary lipids and promotes cholesterol crystal nucleation in model bile

BF Smith
Section of Gastroenterology, University Hospital, Boston, MA.

The binding of phosphatidylcholine and cholesterol in model bile to human gallbladder mucin was studied by means of a rapid filtration binding assay and sucrose density gradient ultracentrifugation. Numerous low affinity binding sites for phosphatidylcholine and cholesterol were present on gallbladder mucin. Binding of phosphatidylcholine and cholesterol to mucin increased as a function of cholesterol saturation index. Proteolytic digestion of mucin disaggregated the native mucin polymer and removed hydrophobic domains on the mucin peptide core that bind l-anilino-8-naphthalenesulfonic acid. Proteolytic digestion also resulted in a 91% and 78% decrease, respectively, in the binding of phosphatidylcholine and cholesterol to mucin. The ability of trypsin-treated and native mucin to promote the nucleation of cholesterol monohydrate crystals was compared in a model bile. The incidence of cholesterol monohydrate crystal nucleation with native mucin was significantly greater at 3 days than with trypsin- treated mucin or controls (P less than 0.001). After 3, 6, and 9 days of incubation, samples containing native mucin contained significantly more crystals than controls or trypsin-digested mucin samples (P less than 0.0001 for each). These data indicate that highly purified human gallbladder mucin binds phosphatidylcholine and cholesterol in model bile. Furthermore, this study demonstrates that structural integrity of the native mucin polymer and hydrophobic domains on the peptide core are essential for the nucleation of cholesterol monohydrate crystals by mucin in model bile.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. Finzi, V. Barbu, P.-R. Burgel, M. Mergey, K. S. Kirkwood, E. C. Wick, J.-Y. Scoazec, F. Peschaud, F. Paye, J. A. Nadel, et al. MUC5AC, a Gel-Forming Mucin Accumulating in Gallstone Disease, Is Overproduced via an Epidermal Growth Factor Receptor Pathway in the Human Gallbladder Am. J. Pathol., December 1, 2006; 169(6): 2031 - 2041. [Abstract] [Full Text] [PDF]

				R A Kozarek Pancreaticobiliary biofilm: is cholesterol the answer? Gut, March 1, 2005; 54(3): 326 - 328. [Full Text] [PDF]

				P. Portincasa, N. G. Venneman, A. Moschetta, A. van den Berg, G. Palasciano, G. P. vanBerge-Henegouwen, and K. J. van Erpecum Quantitation of cholesterol crystallization from supersaturated model bile J. Lipid Res., April 1, 2002; 43(4): 604 - 610. [Abstract] [Full Text] [PDF]

				R. Kuver, J. H. Klinkspoor, W. R. A. Osborne, and S. P. Lee Mucous granule exocytosis and CFTR expression in gallbladder epithelium Glycobiology, February 1, 2000; 10(2): 149 - 157. [Abstract] [Full Text] [PDF]

				D. Q-H. Wang, D. E. Cohen, F. Lammert, and M. C. Carey No pathophysiologic relationship of soluble biliary proteins to cholesterol crystallization in human bile J. Lipid Res., March 1, 1999; 40(3): 415 - 425. [Abstract] [Full Text]

				B. J.-W. Van Klinken, J. Dekker, S. A. Van Gool, J. Van Marle, H. A. Buller, and A. W. C. Einerhand MUC5B is the prominent mucin in human gallbladder and is also expressed in a subset of colonic goblet cells Am J Physiol Gastrointest Liver Physiol, May 1, 1998; 274(5): G871 - G878. [Abstract] [Full Text] [PDF]