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Journal of Lipid Research, Vol 29, 102-108, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Comparison of commercial kits for apoprotein A-I and apoprotein B with standardized apoprotein A-I and B radioimmunoassays performed at the Northwest Lipid Research Center

JJ Albers and JL Adolphson
Department of Medicine and Pathology, University of Washington School of Medicine, Seattle 98104.

There has recently been a proliferation of commercial kits available for apoproteins A-I and B. Since reference procedures for apoproteins have not yet been established we have elected to compare apoprotein kit methods with highly standardized apoprotein B and A-I radioimmunoassays developed at the Northwest Lipid Research Center. Commercial radial immunodiffusion kits for apoproteins A-I and B were obtained from three separate companies, Calbiochem, Daiichi Pure Chemicals, and Tago, and a commercial radioimmunoassay kit for apoprotein A-I was obtained from Ventrex Laboratories. Considerable differences were observed between the commercial kit methods and the Northwest Lipid Research radioimmunoassay methods. Some of the differences between methods were related to the assigned value of the reference materials. Other differences between methods were clearly method-dependent.
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