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Journal of Lipid Research, Vol 29, 109-112, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Determination of phospholipid base structure by CA MIKES mass spectrometry

C Easton, DW Johnson and A Poulos
Department of Organic Chemistry, University of Adelaide, South Australia.

The fast atom bombardment spectra of three phospholipids containing a secondary, tertiary, and quaternary nitrogenous base attached to a 1,2- dipalmitoyl-sn-glycero-3-phosphate skeleton were shown to each contain an ion derived from the intact nitrogenous base. The collisional activation mass analyzed ion kinetic energy spectroscopy (CA MIKES) spectrum produced from each of these ions was shown to be unique for the particular base. The technique thus provides a non-destructive rapid identification of the phosphorylated base of phospholipids.
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