HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Coleman, R. A. Articles by Davis, R. A. Search for Related Content PubMed PubMed Citation Articles by Coleman, R. A. Articles by Davis, R. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 29, 33-42, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Developmental coordinate expression of triacylglycerol and small molecular weight apoB synthesis and secretion by rat hepatocytes

RA Coleman, EB Haynes, TM Sand and RA Davis
Department of Pediatrics, Duke University Medical Center, Durham, NC 27710.

Glycerolipid and apoB synthesis and secretion were examined in hepatocytes obtained from fetal, suckling (day 6), and adult rats in order to examine the developmental regulation of lipoprotein production. The capacity to synthesize [3H]triacylglycerol (from [3H]glycerol) followed the order: adult greater than day 6 greater than fetal. Addition of 1 mM oleic acid to the incubation media stimulated the incorporation of [3H]glycerol into triacylglycerol 6.7- and 3.6- fold by fetal and adult hepatocytes, respectively. After maximal stimulation by 1 mM oleic acid, triacylglycerol secretion by fetal cells was still only 39% of the amount secreted by adult cells that had been treated similarly. Fetal cells stimulated with 1 mM oleic acid synthesized the same amount of triacylglycerol as adult cells that had been treated with 0.1 mM oleic acid. However, the fetal cells secreted only one-third as much triacylglycerol, further demonstrating relatively impaired secretion of triacylglycerol. In order to determine whether low triacylglycerol secretion was associated with differences in apoB metabolism, cells were incubated with [35S]methionine and apoB was quantified after immunoprecipitation. Fetal cells synthesized and secreted nearly equal amounts of large molecular weight and small molecular weight apoB. In contrast, adult cells synthesized and secreted nearly twice as much small molecular weight apoB as large molecular weight apoB. Moreover, although fetal and adult cells secreted large molecular weight apoB at similar rates, adult cells synthesized and secreted small molecular weight apoB at rates that were nearly two times higher than fetal cells. These data suggest that the ability to assemble and secrete VLDL varies in parallel with the developmental expression of small molecular weight apoB. These studies also show the usefulness of the cultured rat hepatocyte model for examining the ontogeny and regulation of lipoprotein assembly/secretion.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. J. Waterman, N. T. Price, and V. A. Zammit Distinct ontogenic patterns of overt and latent DGAT activities of rat liver microsomes J. Lipid Res., September 1, 2002; 43(9): 1555 - 1562. [Abstract] [Full Text] [PDF]

				D. Plonne, H.-P. Schulze, U. Kahlert, K. Meltke, H. Seidolt, A. J. Bennett, I. J. Cartwright, J. A. Higgins, U. Till, and R. Dargel Postnatal development of hepatocellular apolipoprotein B assembly and secretion in the rat J. Lipid Res., November 1, 2001; 42(11): 1865 - 1878. [Abstract] [Full Text] [PDF]

				J. I. Trickett, D. D. Patel, B. L. Knight, E. D. Saggerson, G. F. Gibbons, and R. J. Pease Characterization of the Rodent Genes for Arylacetamide Deacetylase, a Putative Microsomal Lipase, and Evidence for Transcriptional Regulation J. Biol. Chem., October 19, 2001; 276(43): 39522 - 39532. [Abstract] [Full Text] [PDF]

				D. Linden, A. Sjoberg, L. Asp, L. Carlsson, and J. Oscarsson Direct effects of growth hormone on production and secretion of apolipoprotein B from rat hepatocytes Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1335 - E1346. [Abstract] [Full Text] [PDF]

				S.-L. Wang, E. Z. Du, T. D. Martin, and R. A. Davis Coordinate Regulation of Lipogenesis, the Assembly and Secretion of Apolipoprotein B-containing Lipoproteins by Sterol Response Element Binding Protein 1 J. Biol. Chem., August 1, 1997; 272(31): 19351 - 19358. [Abstract] [Full Text] [PDF]

				P. J. Coussons, C. S. Bourgeois, D. Wiggins, and G. F. Gibbons Selective Recruitment of ApoB-48 for the Assembly of VLDL in Rat Triacylglycerol-Enriched Hepatocytes Arterioscler. Thromb. Vasc. Biol., July 1, 1996; 16(7): 889 - 897. [Abstract] [Full Text]

				D. N. Douglas, V. W. Dolinsky, R. Lehner, and D. E. Vance A Role for Sp1 in the Transcriptional Regulation of Hepatic Triacylglycerol Hydrolase in the Mouse J. Biol. Chem., June 29, 2001; 276(27): 25621 - 25630. [Abstract] [Full Text] [PDF]