HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Crawford, J. M. Articles by Gollan, J. L. Search for Related Content PubMed PubMed Citation Articles by Crawford, J. M. Articles by Gollan, J. L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 29, 144-156, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Role of the hepatocyte microtubular system in the excretion of bile salts and biliary lipid: implications for intracellular vesicular transport

JM Crawford, CA Berken and JL Gollan
Department of Pathology, Harvard Medical School, Boston, MA.

The role of the hepatocyte microtubular system in the transport and excretion of bile salts and biliary lipid has not been defined. In this study the effects of microtubule inhibition on biliary excretion of micelle- and non-micelle-forming bile salts and associated lipid were examined in rats. Low-dose colchicine pretreatment had no effect on the baseline excretion of biliary bile salts and phospholipid in animals studied 1 hr after surgery (basal animals), but slightly retarded the excretion of tracer [14C]taurocholate relative to that of lumicolchicine-pretreated (control) rats. However, colchicine pretreatment resulted in a marked reduction in the excretion of 2 mumol/100 g doses of a series of four micelle-forming bile salts of differing hydrophilicity, but had no significant effect on the excretion of the non-micelle-forming bile salt, taurodehydrocholate. Continuous infusion of 0.2 mumol of taurocholate/(100 g.min) following 24 hr of biliary drainage (depleted/reinfused animals) resulted in physiologic bile flow with biliary excretion rates of bile salts, phospholipid, and cholesterol that were markedly inhibited (mean 33, 39, and 42%, respectively) by colchicine or vinblastine pretreatment. Excretion of tracer [14C]taurocholate also was markedly delayed by colchicine in these bile salt-depleted/reinfused animals. In contrast, colchicine did not inhibit bile salt excretion in response to reinfusion of taurodehydrocholate. Thus, under basal conditions, the microtubular system appears to play a minor role in hepatic transport and excretion of bile salts and biliary lipid. However, biliary excretion of micelle-forming bile salts and associated phospholipid and cholesterol becomes increasingly dependent on microtubular integrity as the transcellular flux and biliary excretion of bile salts increases, in both bile salt-depleted and basal animals. We postulate that cotransport of micelle-forming bile salts and lipids destined for biliary excretion, via an intracellular vesicular pathway, forms the basis for this microtubule dependence.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. J. Snooks, P. Bhat, J. Mackenzie, N. A. Counihan, N. Vaughan, and D. A. Anderson Vectorial Entry and Release of Hepatitis A Virus in Polarized Human Hepatocytes J. Virol., September 1, 2008; 82(17): 8733 - 8742. [Abstract] [Full Text] [PDF]

				M. K. Wu, H. Hyogo, S. K. Yadav, P. M. Novikoff, and D. E. Cohen Impaired response of biliary lipid secretion to a lithogenic diet in phosphatidylcholine transfer protein-deficient mice J. Lipid Res., March 1, 2005; 46(3): 422 - 431. [Abstract] [Full Text] [PDF]

				S. Misra, P. Ujhazy, Z. Gatmaitan, L. Varticovski, and I. M. Arias The Role of Phosphoinositide 3-Kinase in Taurocholate-induced Trafficking of ATP-dependent Canalicular Transporters in Rat Liver J. Biol. Chem., October 9, 1998; 273(41): 26638 - 26644. [Abstract] [Full Text] [PDF]

				S. F. HAMM-ALVAREZ and M. P. SHEETZ Microtubule-Dependent Vesicle Transport: Modulation of Channel and Transporter Activity in Liver and Kidney Physiol Rev, October 1, 1998; 78(4): 1109 - 1129. [Abstract] [Full Text] [PDF]