Journal of Lipid Research, Vol 29, 227-235, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Specific methylation of plasma nonesterified fatty acids in a one-step reaction

G Lepage and CC Roy
Pediatric Gastroenterology Unit, Hopital Ste-Justine, Montreal, Quebec, Canada.

Analysis of plasma nonesterified fatty acids (NEFA) by gas-liquid chromatography requires procedures that are both lengthy and cumbersome. A 45-min direct methylation procedure was carried out at 24- 29 degrees C on 150 microliter of plasma added with an internal standard in 5.0 ml of methanol-acetyl chloride 50:1 (v/v). To stop the reaction, 3 ml of 6.0% K2CO3 was added. After addition of 150 microliter of hexane, shaking and centrifugation, an aliquot of the upper phase was injected into the gas chromatograph. The specificity of the methylation reaction for NEFA without hydrolysis of other classes of plasma lipids was substantiated with appropriate standards. This one- step specific methylation procedure is superior to currently used methods.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. S. Bradley, G. J. F. Heigenhauser, B. D. Roy, E. M. Staples, J. G. Inglis, P. J. LeBlanc, and S. J. Peters The acute effects of differential dietary fatty acids on human skeletal muscle pyruvate dehydrogenase activity J Appl Physiol, January 1, 2008; 104(1): 1 - 9. [Abstract] [Full Text] [PDF]

				A. M Hill, J. D Buckley, K. J Murphy, and P. R. Howe Combining fish-oil supplements with regular aerobic exercise improves body composition and cardiovascular disease risk factors Am. J. Clinical Nutrition, May 1, 2007; 85(5): 1267 - 1274. [Abstract] [Full Text] [PDF]

				A. Al-Khalifa, T. G. Maddaford, M. N. Chahine, J. A. Austria, A. L. Edel, M. N. Richard, B. P. Ander, N. Gavel, M. Kopilas, R. Ganguly, et al. Effect of dietary hempseed intake on cardiac ischemia-reperfusion injury Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1198 - R1203. [Abstract] [Full Text] [PDF]

				J. W. Gaubatz, B. K. Gillard, J. B. Massey, R. C. Hoogeveen, M. Huang, E. E. Lloyd, J. L. Raya, C.-y. Yang, and H. J. Pownall Dynamics of dense electronegative low density lipoproteins and their preferential association with lipoprotein phospholipase A2 J. Lipid Res., February 1, 2007; 48(2): 348 - 357. [Abstract] [Full Text] [PDF]

				D. J. Baer, J. T. Judd, P. M. Kris-Etherton, G. Zhao, and E. A. Emken Stearic Acid Absorption and Its Metabolizable Energy Value Are Minimally Lower than Those of Other Fatty Acids in Healthy Men Fed Mixed Diets J. Nutr., December 1, 2003; 133(12): 4129 - 4134. [Abstract] [Full Text] [PDF]

				H. G. Wahl, C. Kausch, F. Machicao, K. Rett, M. Stumvoll, and H.-U. Haring Troglitazone Downregulates {Delta}-6 Desaturase Gene Expression in Human Skeletal Muscle Cell Cultures Diabetes, April 1, 2002; 51(4): 1060 - 1065. [Abstract] [Full Text] [PDF]

				J. G. Richards, G. J. F. Heigenhauser, and C. M. Wood Lipid oxidation fuels recovery from exhaustive exercise in white muscle of rainbow trout Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2002; 282(1): R89 - R99. [Abstract] [Full Text] [PDF]

				S. Yu-Poth, T. D. Etherton, C. C. Reddy, T. A. Pearson, R. Reed, G. Zhao, S. Jonnalagadda, Y. Wan, and P. M. Kris-Etherton Lowering Dietary Saturated Fat and Total Fat Reduces the Oxidative Susceptibility of LDL in Healthy Men and Women J. Nutr., September 1, 2000; 130(9): 2228 - 2237. [Abstract] [Full Text]

				F. Enjalbert, M.-C. Nicot, C. Bayourthe, and R. Moncoulon Duodenal Infusions of Palmitic, Stearic or Oleic Acids Differently Affect Mammary Gland Metabolism of Fatty Acids in Lactating Dairy Cows J. Nutr., September 1, 1998; 128(9): 1525 - 1532. [Abstract] [Full Text]