J. Lipid Res.
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Journal of Lipid Research, Vol 29, 235-239, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Inhibitors of sterol synthesis. Reverse phase high performance liquid chromatography for the separation of cholesterol, 5 alpha-cholest-8(14)- en-3 beta-ol-15-one, and their fatty acid esters

A Chu and GJ Schroepfer Jr
Department of Biochemistry, Rice University, Houston, TX 77251.

A relatively simple and rapid method was required for the separation of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one, a potent inhibitor of sterol synthesis, from its major metabolites. Conditions have been determined which permit the resolution of the 15-ketosterol and cholesterol and fatty acid esters of the two sterols by reverse phase high performance chromatography. This methodology also permits the resolution of the major esters of the 15-ketosterol and of cholesterol.
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G. J. Schroepfer Jr.
Oxysterols: Modulators of Cholesterol Metabolism and Other Processes
Physiol Rev, January 1, 2000; 80(1): 361 - 554.
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