J. Lipid Res.
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Journal of Lipid Research, Vol 29, 532-537, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis

K Prydz, BF Kase and JI Pedersen
Institute for Nutrition Research, School of Medicine, University of Oslo, Norway.

Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol. Four different mobile phases (water-methanol, 10 mM acetate buffer (pH 4.37)- methanol, 30 mM trifluoroacetic acid (pH 2.9 with triethylamine)- methanol, and 50 mM potassium phosphate buffer (pH 7.0)-2-propanol) have been applied to obtain separation of all the main intermediates with use of the same reversed phase column (Zorbax ODS).
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