Journal of Lipid Research, Vol 29, 629-641, Copyright © 1988 by Lipid Research, Inc.

ARTICLES

Occurrence of 3 beta-hydroxy-5-cholestenoic acid, 3 beta,7 alpha- dihydroxy-5-cholestenoic acid, and 7 alpha-hydroxy-3-oxo-4-cholestenoic acid as normal constituents in human blood

M Axelson, B Mork and J Sjovall
Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden.

Three unconjugated C27 bile acids were found in plasma from healthy humans. They were isolated by liquid-solid extraction and anion- exchange chromatography and were identified by gas-liquid chromatography-mass spectrometry, microchemical reactions, and ultraviolet spectroscopy as 3 beta-hydroxy-5-cholestenoic, 3 beta,7 alpha-dihydroxy-5-cholestenoic, and 7 alpha-hydroxy-3-oxo-4- cholestenoic acids. Their levels often exceeded those of the unconjugated C24 bile acids and the variations between individuals were smaller than for the C24 acids. The concentrations in plasma from 11 healthy subjects were 67.2 +/- 27.9 ng/ml (mean +/- SD) for 3 beta- hydroxy-5-cholestenoic acid, 38.9 +/- 25.6 ng/ml for 3 beta,7 alpha- dihydroxy-5-cholestenoic acid, and 81.7 +/- 27.9 ng/ml for 7 alpha- hydroxy-3-oxo-4-cholestenoic acid. The levels of the individual acids were positively correlated to each other and not to the levels of the C24 acids. The cholestenoic acids were below the detection limit (20-50 ng/ml) in bile and C27 bile acids present in bile were not detected in plasma.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Meaney, M. Heverin, U. Panzenboeck, L. Ekstrom, M. Axelsson, U. Andersson, U. Diczfalusy, I. Pikuleva, J. Wahren, W. Sattler, et al. Novel route for elimination of brain oxysterols across the blood-brain barrier: conversion into 7{alpha}-hydroxy-3-oxo-4-cholestenoic acid J. Lipid Res., April 1, 2007; 48(4): 944 - 951. [Abstract] [Full Text] [PDF]

				M. Andersson, L. Ellegard, and H. Andersson Oat bran stimulates bile acid synthesis within 8 h as measured by 7{alpha}-hydroxy-4-cholesten-3-one Am. J. Clinical Nutrition, November 1, 2002; 76(5): 1111 - 1116. [Abstract] [Full Text] [PDF]

				J. Y. L. Chiang Bile Acid Regulation of Gene Expression: Roles of Nuclear Hormone Receptors Endocr. Rev., August 1, 2002; 23(4): 443 - 463. [Abstract] [Full Text] [PDF]

				I. Björkhem, L. Starck, U. Andersson, D. Lütjohann, S. von Bahr, I. Pikuleva, A. Babiker, and U. Diczfalusy Oxysterols in the circulation of patients with the Smith-Lemli-Opitz syndrome: abnormal levels of 24S- and 27-hydroxycholesterol J. Lipid Res., March 1, 2001; 42(3): 366 - 371. [Abstract] [Full Text]

				A. Babiker, O. Andersson, D. Lindblom, J. van der Linden, B. Wiklund, D. Lütjohann, U. Diczfalusy, and I. Björkhem Elimination of cholesterol as cholestenoic acid in human lung by sterol 27-hydroxylase: evidence that most of this steroid in the circulation is of pulmonary origin J. Lipid Res., August 1, 1999; 40(8): 1417 - 1425. [Abstract] [Full Text]

				W. C. Duane and N. B. Javitt 27-Hydroxycholesterol: production rates in normal human subjects J. Lipid Res., July 1, 1999; 40(7): 1194 - 1199. [Abstract] [Full Text]

				I. A. Pikuleva, A. Babiker, M. R. Waterman, and I. Bjorkhem Activities of Recombinant Human Cytochrome P450c27 (CYP27) Which Produce Intermediates of Alternative Bile Acid Biosynthetic Pathways J. Biol. Chem., July 17, 1998; 273(29): 18153 - 18160. [Abstract] [Full Text] [PDF]

				E. Lund, O. Andersson, J. Zhang, A. Babiker, G. Ahlborg, U. Diczfalusy, K. Einarsson, J. Sjovall, and I. Bjorkhem Importance of a Novel Oxidative Mechanism for Elimination of Intracellular Cholesterol in Humans Arterioscler. Thromb. Vasc. Biol., February 1, 1996; 16(2): 208 - 212. [Abstract] [Full Text]