| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 29, 679-689, Copyright © 1988 by Lipid Research, Inc.
P Meneses and T Glonek
The common phospholipids from biological sources were quantitated using
phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy in conjunction
with an analytical reagent composed of two parts: 1) 2 ml of reagent
chloroform in which was dissolved 0.01-100 mg of crude tissue lipid
extracted from tissue sources using chloroform-methanol 2:1, the extract
having been washed with 0.2 vol. of 0.1 M KCl; 2) 1 ml of an aqueous
methanol reagent composed of one part 0.2 M (ethylenedinitrilo)-tetraacetic
acid in D2O titrated to pH 6 with CsOH and four parts of reagent methanol.
In a magnetic field of 11.75 Tesla, the extracted phospholipids yield
narrow signals (1.8-3.2 Hz at half- height), corresponding to each generic
species, e.g., phosphatidylcholines, phosphatidylethanolamines, etc.,
permitting resolution among the various phospholipid families and their
lyso and plasmalogen derivatives. The reagent permits assays of high
precision and accuracy using a modest amount of NMR spectrometer time (ca.
15 min/assay). The procedures described, which are compared to high-
performance liquid chromatography, are convenient for the routine analysis
of phospholipids from biological sources.
ARTICLES
High resolution 31P NMR of extracted phospholipids
MR Laboratory, Chicago College of Osteopathic Medicine, IL 60615.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Renner, B. Kessler, and D. Oesterhelt Lipid composition of integral purple membrane by 1H and 31P NMR J. Lipid Res., August 1, 2005; 46(8): 1755 - 1764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rujoi, J. Jin, D. Borchman, D. Tang, and M. C. Yappert Isolation and Lipid Characterization of Cholesterol-Enriched Fractions in Cortical and Nuclear Human Lens Fibers Invest. Ophthalmol. Vis. Sci., April 1, 2003; 44(4): 1634 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Katz-Brull, D. Seger, D. Rivenson-Segal, E. Rushkin, and H. Degani Metabolic Markers of Breast Cancer: Enhanced Choline Metabolism and Reduced Choline-Ether-Phospholipid Synthesis Cancer Res., April 1, 2002; 62(7): 1966 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Corcelli, V. M. T. Lattanzio, G. Mascolo, P. Papadia, and F. Fanizzi Lipid-protein stoichiometries in a crystalline biological membrane: NMR quantitative analysis of the lipid extract of the purple membrane J. Lipid Res., January 1, 2002; 43(1): 132 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sterin, J. S. Cohen, Y. Mardor, E. Berman, and I. Ringel Levels of Phospholipid Metabolites in Breast Cancer Cells Treated with Antimitotic Drugs: A 31P-Magnetic Resonance Spectroscopy Study Cancer Res., October 1, 2001; 61(20): 7536 - 7543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Gout, S. Aubert, R. Bligny, F. Rébeillé, A. R. Nonomura, A. A. Benson, and R. Douce Metabolism of Methanol in Plant Cells. Carbon-13 Nuclear Magnetic Resonance Studies Plant Physiology, May 1, 2000; 123(1): 287 - 296. [Abstract] [Full Text]
|
|
|
|
|
|
B. Moesgaard, J. W. Jaroszewski, and H. S. Hansen Accumulation of N-acyl-ethanolamine phospholipids in rat brains during post-decapitative ischemia: a 31P NMR study J. Lipid Res., March 1, 1999; 40(3): 515 - 521. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Chi, D. M. de Freitas, M. Sikora, and V. K. Bansal Correlations of Na+-Li+ Exchange Activity With Na+ and Li+ Binding and Phospholipid Composition in Erythrocyte Membranes of White Hypertensive and Normotensive Individuals : A Nuclear Magnetic Resonance Investigation Hypertension, March 1, 1996; 27(3): 456 - 464. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
