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Journal of Lipid Research, Vol 29, 691-697, Copyright © 1988 by Lipid Research, Inc.


ARTICLES

Unilocular fat cells in three-dimensional collagen gel matrix culture

H Sugihara, N Yonemitsu, S Toda, S Miyabara, S Funatsumaru and T Matsumoto
Department of Pathology, Saga Medical School, Japan.

Three-dimensional culture with collagen gel, developed recently for the in vitro study of some mammalian cells in a more physiological condition than a monolayer culture, was applied for a biological study of unilocular fat cells. Successfully embedded in the gel, the unilocular fat cells were shown to be able to keep their cellular function and actively proliferate. These findings confirm that unilocular fat cells do undergo proliferation under in vitro conditions as demonstrated in monolayer culture.
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