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Journal of Lipid Research, Vol 29, 755-764, Copyright © 1988 by Lipid Research, Inc.
C Lenich, P Brecher, S Makrides, A Chobanian and VI Zannis
We have used human apolipoprotein cDNAs as hybridization probes to study
the relative abundance and distribution of apolipoprotein mRNAs in rabbit
tissues by RNA blotting analysis. The tissues surveyed included liver,
intestine, lung, pancreas, spleen, stomach, skeletal muscle, testis, heart,
kidney, adrenal, aorta, and brain. We found that liver is the sole or major
site of synthesis of apoA-II, apoA-IV, apoB, apoC-I, apoC-II, apoC-III, and
apoE, and the intestine is a major site of synthesis of apoA-I, apoA-IV,
and apoB. Minor sites of apolipoprotein mRNA synthesis were as follows:
apoA-I, liver and skeletal muscle; apoA-IV, spleen and lung; apoB, kidney;
apoC-II and apoC-III, intestine. ApoE mRNA was detected in all tissues
surveyed with the exception of skeletal muscle. Sites with moderate apoE
mRNA (10% of the liver value) were lung, brain, spleen, stomach, and
testis. All rabbit mRNAs had forms with sizes comparable to their human
counterparts. In addition, hybridization of hepatic and intestinal RNA with
human apoA-IV and apoB probes produced a second hybridization band of
approximately 2.4 and 8 kb, respectively. Similarly, hybridization of
rabbit intestinal RNA with human apoC-II produced a hybridization band of
1.8 kb. The 8 kb apoB mRNA form may correspond to the apoB-48 mRNA, whereas
the apoA-IV- and apoC-II-related mRNA species have not been described
previously. This study provides a comprehensive survey of the sites of
apolipoprotein gene expression and shows numerous differences in both the
abundance and the tissue distribution of several apolipoprotein mRNAs
between rabbit and human tissues. These findings and the observation of
potentially new apolipoprotein mRNA species are important for our
understanding of the cis and trans acting factors that confer tissue
specificity as well as factors that regulate the expression of
apolipoprotein genes in different mammalian species.
ARTICLES
Apolipoprotein gene expression in the rabbit: abundance, size, and distribution of apolipoprotein mRNA species in different tissues
Department of Medicine, Boston University Medical Center, MA 02118.
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