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Journal of Lipid Research, Vol 29, 925-936, Copyright © 1988 by Lipid Research, Inc.
JS Cohn, JR McNamara, SD Cohn, JM Ordovas and EJ Schaefer
Twenty two subjects (9 males, 13 females) were fed a fat-rich meal (1 g of
fat/kg body weight). Triglyceride-rich lipoproteins (TRL) were isolated by
ultracentrifugation (d less than 1.006 g/ml) from blood drawn 0, 3, 6, 9,
and 12 hr after the meal. Plasma triglyceride increased then decreased
postprandially, while plasma apoA-I and apoB concentrations decreased. TRL
triglyceride, TRL total protein, and TRL apoB concentrations all increased
then decreased after the fat-rich meal. Postprandial rise in plasma
triglyceride was significantly correlated with fasting plasma triglyceride
levels (r = 0.66, P less than 0.001); postprandial rise in TRL triglyceride
was significantly correlated with fasting TRL triglyceride levels (r =
0.58, P less than 0.01); postprandial rise in TRL apoB was not, however,
significantly correlated with fasting TRL apoB levels (r = 0.37, N.S.). TRL
apolipoproteins were separated by polyacrylamide gradient (4-22.5%) gel
electrophoresis and protein bands were scanned in two dimensions with a
laser densitometer. Relative postprandial changes in the concentration of
the TRL apolipoproteins were determined. TRL apoB-100, apoB-48, apoE, and
apoC increased then decreased postprandially. The increase in TRL apoB-100
after the fat-rich meal was confirmed in 8 subjects by direct measurement
of apoB-100 with a monoclonal antibody ELISA assay. ApoA-I concentration in
TRL was unchanged. Albumin in the TRL fraction was significantly increased
12 hr after the meal. Subjects with a greater magnitude of postprandial
triglyceridemia had a greater increase in TRL triglyceride and TRL apoB,
but their TRL apoB-100/apoB- 48 ratios were not different from subjects
with less pronounced triglyceridemia. Assuming that plasma TRL containing
apoB-100 are predominantly derived from the liver, our data suggest that
triglyceride-rich lipoproteins from both the liver and intestine make a
significant contribution to postprandial triglyceridemia.
ARTICLES
Plasma apolipoprotein changes in the triglyceride-rich lipoprotein fraction of human subjects fed a fat-rich meal
Lipid Metabolism Laboratory, USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111.
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